Mechanisms of genomic stability maintenance by replication scaffold protein, AND-1

DNA复制、检查点激活和复制后染色单体凝聚力的协调执行与复制分叉机制密切相关。人类AND-1定位于复制支架上，是有效合成DNA所必需的，其缺失会增加DNA损伤，延缓S期的进展，导致癌细胞死亡。复制胁迫中检查点的激活、UV-RDS的抑制和同源重组修复也需要And-1。本研究表明，And-1在S期和G2期协调多个细胞事件。

Coordinated execution of DNA replication, checkpoint activation and postreplicative chromatid cohesion is intimately related to the replication fork machinery. Human AND-1 is localized at the replication scaffold, and is required for efficient DNA synthesis and its depletion increases DNA damage, delays progression of S phase, leads to cell death in cancer cells. AND-1 is also required for checkpoint activation in replication stress, inhibition of UV-RDS and homologous recombinational repair. This study indicates that AND-1 coordinates multiple cellular events in S phase and G2 phase.