Mechanisms of genomic stability maintenance by replication scaffold protein, AND-1

复制支架蛋白 AND-1 维持基因组稳定性的机制

• 批准号: 20570173
• 负责人: YOSHIZAWA Naoko
• 金额: $ 3.08万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570173/
• 关键词: DNA複製; 複製チェックポイント; 複製フォーク; 複製ストレス; DNA修復; ゲノム不安定性; 姉妹染色体交換; AND-1; Timeless; 修復組換え; ゲノム安定性; 染色体接着; コヒーシン; 相同組換え修復; DNA損傷

Coordinated execution of DNA replication, checkpoint activation and postreplicative chromatid cohesion is intimately related to the replication fork machinery. Human AND-1 is localized at the replication scaffold, and is required for efficient DNA synthesis and its depletion increases DNA damage, delays progression of S phase, leads to cell death in cancer cells. AND-1 is also required for checkpoint activation in replication stress, inhibition of UV-RDS and homologous recombinational repair. This study indicates that AND-1 coordinates multiple cellular events in S phase and G2 phase.

DNA复制、检查点激活和复制后染色单体内聚的协调执行与复制叉机制密切相关。人类的and -1定位于复制支架，是有效的DNA合成所必需的，它的耗尽会增加DNA损伤，延迟S期的进展，导致癌细胞的细胞死亡。在复制应激中，检查点激活、抑制UV-RDS和同源重组修复也需要and -1。本研究表明，and -1在S期和G2期协调多种细胞事件。

项目成果

Regulation of replication fork integrity during S phase.

S 期复制叉完整性的调节。

• 发表时间: 2008
• 作者: Hisao Masai;Motoshi Hayano;Seiji Matsumoto;Yutaka Kanoh;Mika Yokoyama;Michiyo Shimmoto;Naoko Kakusho;Naoko Yoshizawa;Taku Tanaka
• 通讯作者: Taku Tanaka

Roles of human replisome component AND-1 and Tim in S phase chro-mosome transaction

人类复制体组件AND-1和Tim在S期染色体交易中的作用

• 发表时间: 2010
• 作者: Naoko Yoshizawa;Hisao Masai
• 通讯作者: Hisao Masai

Regulation of replication program and maintenance of genetic integrity.

复制程序的调节和遗传完整性的维护。

• 发表时间: 2010
• 作者: Masai;H.;Yamazaki;S.;Hayano;M.;Kanoh;Y.;Matsumoto;S.;Kakusho;N.;Ishii;A.;Shimmoto;M;Oda;M.;Yoshizawa;N.;Ito;S.;Kudo;S.(Organizer and speaker)
• 通讯作者: S.(Organizer and speaker)

Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombination repair

人类 AND-1 是高效 DNA 复制、检查点激活和同源重组修复所必需的

• 发表时间: 2008
• 作者: Naoko Yoshizawa-Sugata;Hisao Masai
• 通讯作者: Hisao Masai

Eukaryotic DNA replication; where, when and how?

真核DNA复制；

• 发表时间: 2010
• 期刊: Annual Rev.Biochem. 79
• 作者: Masai;H.;Matsumoto;S.;You;Z.;Yoshizawa-Sugata;N.;Oda;M.
• 通讯作者: M.