Evaluation of freeze-dried bull spermatozoa by intra-or inter-species micro insemination

通过种内或种间显微授精评价冻干公牛精子

• 批准号: 20580305
• 负责人: HOCHI Shinichi
• 金额: $ 2.91万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580305/
• 关键词: ウシ精子; 顕微授精; 凍結乾燥; カルシウムオシレーション; DNA脱メチル化; ROCK阻害剤; ガラス化保存; 微小管形成中心; 精子中心体; コメットアッセイ; 胚盤胞; 共焦点レーザー顕微鏡; 超急速ガラス化保存; 卵子活性化

The purpose of this study was to investigate whether freeze-drying process of bull spermatozoa disturbed early post-fertilization events essential to gain the developmental competence of intracytoplasmically sperm-injected(ICSI) oocytes. In interspecies assay using mouse ovulated oocytes, the ability of freeze-dried(FD) bull spermatozoa to induce normal intracellular calcium oscillation pattern was comparable despite of temperatures stored for 1 year. DNA fragmentation in FD spermatozoa stored at+ 4℃was not detected by alkaline comet assay. With intraspecies(bovine-bovine) ICSI, the FD process was found to be innocent in performance of epigenetic remodeling(active DNA demethylation) and function of microtubule-organizing center(sperm aster formation and network assembly) in the pronuclear-stage zygotes. Factors responsible for low blastocyst yield from the FD-ICSI bovine oocytes remain to be determined.

这项研究的目的是研究牛牛精子的冻干过程是否影响了早期的施肥后事件，这对于获得了室内注入精子（ICSI）卵母细胞的发育能力所必需的。在使用小鼠排卵卵母细胞的种间测定中，冷冻干燥（FD）牛精子诱导正常细胞内钙振荡模式的能力是存储1年温度的可比任务。酒精彗星分析未检测到储存在+ 4的FD精子中的DNA碎片化。伴有播质（牛 - 牛）ICSI，发现FD过程在表观遗传重塑（活性DNA脱甲基化）的性能和微管 - 组织中心（精子Aster形成和网络组装）的功能方面是无辜的。造成FD-ISI牛卵母细胞胚泡低产量的低胚泡产量的因素仍有待确定。

项目成果

Stimulatory effect of Rho-associated coiled-coil kinase(ROCK)inhibitor on revivabilitv of in vitro-produced bovine blastocysts after vitrification

Rho相关卷曲螺旋激酶(ROCK)抑制剂对体外牛囊胚玻璃化冷冻后复苏的促进作用

• 发表时间: 2010
• 期刊: Theriogenology
• 影响因子: 2.8
• 作者: S.Hochi;H.Abdalla;H;Hara;M。Shimoda;H.Morita;M.Kuwayama;M.Hirabayashi.;Shinichi Hochi
• 通讯作者: Shinichi Hochi

Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa

• DOI: 10.1262/jrd.11-061o
• 发表时间: 2011-10-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Hochi, Shinichi;Abdalla, Hany;Hirabayashi, Masumi
• 通讯作者: Hirabayashi, Masumi

Demethylation dynamics of paternal genomes in pronuclear bovine zygotes produced by IVF and ICSI with non-dried or freeze-dried spermatozoa

使用非干燥或冻干精子通过 IVF 和 ICSI 产生的原核牛受精卵中父本基因组的去甲基化动态

• 发表时间: 2009
• 作者: Hara;K.;下田美怜;原弘真;下田美怜;保地眞一;Abdalla H;Abdalla H
• 通讯作者: Abdalla H

ウシ卵細胞質内精子注入法の効率改善と凍結乾燥精子を用いた胚盤胞作製の試み

提高牛胞浆内单精子注射效率并尝试利用冻干精子产生囊胚

• 发表时间: 2009
• 作者: Hara;K.;下田美怜;原弘真;下田美怜
• 通讯作者: 下田美怜

In Vitro Fertilization/Intracytoplasmic sperm injection in cattle

牛体外受精/胞浆内单精子注射

• 发表时间: 2011
• 作者: S. Hochi;H. Abdalla;M. Hirabayashi;Shinichi Hochi
• 通讯作者: Shinichi Hochi