Molecular analysis of Rcd1 protein as transcriptional cofactor, which may play a important role in the transformation of muscle cells

Rcd1蛋白作为转录辅助因子的分子分析，其可能在肌肉细胞的转化中发挥重要作用

• 批准号: 20580320
• 负责人: NAGANO Isao
• 金额: $ 3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580320/
• 关键词: 旋毛虫; 筋肉細胞変異; 筋肉細胞分化; Rcd1; AP-1; NF-κB; myogenin; MyoD; 分子生物学; Rcdl

Trichinella spp. infection causes satellite cell proliferation and transformation of muscle cell to the nurse cell. Rcd1 is shown to be a transcriptional cofactor of the c-myb proto-oncogene product. Previously, we cloned Rcd1 gene from the cDNA of T. spiralis muscle larvae, and confirmed that the Rcd1 protein is part of the E-S products. Therefore, the Rcd1 likely affects host cells and tissues for capsule formation. In the present study we investigated the effect by which the Rcd1protein secreted from T. spiralis modulates myogenic differentiation in C2C12 myoblasts, and the role of the Rcd1 protein regulated NF-κB and AP-1 transcriptional pathway. The expression of myogenin and MyoD genes in C2C12 myoblasts transfected with Rcd1 gene was significantly lower than those in non-transfected C2C12 myoblasts. And we demonstrate that Rcd1 is a potent inhibitor of muscle differentiation through activation(C2C12 cells) of NF-κB and AP-1 signaling pathway. It may be inferred from these results that the Rcd1 protein functions as a transcriptional factor or cofactor of satellite cell proliferation and muscle cell transformation.

旋毛虫属感染引起卫星细胞增殖和肌细胞向营养细胞转化。Rcd 1被证明是c-myb原癌基因产物的转录辅因子。本研究首先从T. spiralis肌幼虫的E-S产物，证实了Rcd 1蛋白是E-S产物的一部分。因此，Rcd 1可能影响宿主细胞和组织形成包膜。在本研究中，我们研究了T. spiralis对C2 C12成肌细胞肌源性分化的调节作用，以及Rcd 1蛋白对NF-κB和AP-1转录途径的调节作用。转染Rcd 1基因的C2 C12成肌细胞中myogenin和MyoD基因的表达明显低于未转染的C2 C12成肌细胞。我们证明Rcd 1是通过激活（C2 C12细胞）NF-κB和AP-1信号通路的肌肉分化的有效抑制剂。从这些结果可以推断，Rcd 1蛋白作为转录因子或辅助因子的卫星细胞增殖和肌肉细胞转化的功能。

项目成果

ELISAにおけるTrichinellaの53kDa組換えタンパク質と感染マウス血清との反応性の検討

ELISA检测旋毛虫53kDa重组蛋白与感染小鼠血清的反应性

• 发表时间: 2009
• 作者: Wu;Z.;Nagano;I.;& Takahashi;Y.;Zhiliang Wu;Isao Nagano;長野功;長野功
• 通讯作者: 長野功

Rcd1 protein secreted from Trichinella spiralis down-regulates myogenin and MyoD proteins in C2C12 myoblasts, and inhibits binding activities of transcription factors NF-κB and AP-1

旋毛虫分泌的 Rcd1 蛋白下调 C2C12 成肌细胞中的肌细胞生成素和 MyoD 蛋白，并抑制转录因子 NF-κB 和 AP-1 的结合活性

• 发表时间: 2011
• 作者: Wu;Z.;Nagano;I.;& Takahashi;Y.;Zhiliang Wu;Isao Nagano
• 通讯作者: Isao Nagano

Molecular cloning and characterization of transgelin-like proteins mainly transcribed in newborn larvae of Trichinella spp.

• DOI: 10.1016/j.vetpar.2010.12.031
• 发表时间: 2011-05-31
• 期刊: VETERINARY PARASITOLOGY
• 影响因子: 2.6
• 作者: Nagano, Isao;Wu, Zhiliang;Takahashi, Yuzo
• 通讯作者: Takahashi, Yuzo

Infection of non-encapsulated species of Trichinella ameliorates experimental autoimmune encephalomyelitis involving suppression of Th17 and Th1 response

• DOI: 10.1007/s00436-010-1985-9
• 发表时间: 2010-07
• 期刊: Parasitology Research
• 影响因子: 2
• 作者: Zhiliang Wu;I. Nagano;K. Asano;Y. Takahashi

Resent Advances in Medical Sciences(Parasites and their Human and Animal Hosts)

医学科学最新进展（寄生虫及其人类和动物宿主）

• 发表时间: 2010
• 作者: Wu;Z.;Nagano;I.;& Takahashi;Y.;Zhiliang Wu;Isao Nagano;長野功;長野功;長野功;長野功
• 通讯作者: 長野功