Scientific research of evidence-based prevention of illegal drug of abuse

“指定物质”（Shitei-Yakubutsu）的结构分为三种，即苯乙胺、色胺和哌啶类。建立了多通道电化学高效液相色谱法同时测定指定物质的方法。所提出的在线氧化方法是第一个同时测定各种指定物质的报告。建立了UPLC - FL - ESI-TOF-MS同时测定16种指定物质的方法。但对几种指定物质的荧光检测存在内源性物质干扰。而在实际样品中，采用UPLC分离和TOF-MS检测相结合的方法可以成功地进行测定。该方法已应用于人体血浆、尿液及实物的分析。此外，采用手性试剂R(-)- dbd - pyncs，采用间接手性衍生法对结构中含有不对称碳的苯乙胺进行了对映分离。采用反相高效液相色谱法对12种苯乙胺衍生物进行了高效分离和灵敏检测。该方法已成功地应用于大鼠血浆和实际产品的分析。

The structures of "designated substances" (Shitei-Yakubutsu) are divided into three types, i.e., phenethylamines, tryptamines and piperadines. Simultaneous determination method using HPLC with multi-channel electrochemical detection was developed for the designated substances. The proposed method utilizing online oxidation was the first report on the simultaneous determination of various designated substances. The simultaneous determination based on UPLC with FL detection and ESI-TOF-MS was also developed for 16 designated substances. However, the determination of several designated substances by FL detection was interfered with endogenous substances. However, the determination in real samples was successfully carried out by a combination of UPLC separation and TOF-MS detection. This method was applied to the analyses of human plasma, urine and real products. Furthermore, the enantioseparation of phenethylamines possessing an assymetric carbon in the structure was achieved with indirect chiral derivatization method using a chiral reagent, R(-)-DBD-PyNCS. Twelve kinds of phenethylamine derivatives were efficiently separated by reversed-phase HPLC and sensitively detected. The proposed method was successfully applied to the analyses of rat plasma and real products.