Scientific research of evidence-based prevention of illegal drug of abuse

循证预防非法药物滥用的科学研究

• 批准号: 20590038
• 负责人: TOYOOKA Toshimasa
• 金额: $ 3.08万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590038/
• 关键词: 指定薬物; 液体クロマトグラフィー; 蛍光分析; 質量分析; 電気化学分析; 違法ドラッグ; 光学異性体分析; キラル誘導体化; 高感度特異的分析; 誘導体化

The structures of "designated substances" (Shitei-Yakubutsu) are divided into three types, i.e., phenethylamines, tryptamines and piperadines. Simultaneous determination method using HPLC with multi-channel electrochemical detection was developed for the designated substances. The proposed method utilizing online oxidation was the first report on the simultaneous determination of various designated substances. The simultaneous determination based on UPLC with FL detection and ESI-TOF-MS was also developed for 16 designated substances. However, the determination of several designated substances by FL detection was interfered with endogenous substances. However, the determination in real samples was successfully carried out by a combination of UPLC separation and TOF-MS detection. This method was applied to the analyses of human plasma, urine and real products. Furthermore, the enantioseparation of phenethylamines possessing an assymetric carbon in the structure was achieved with indirect chiral derivatization method using a chiral reagent, R(-)-DBD-PyNCS. Twelve kinds of phenethylamine derivatives were efficiently separated by reversed-phase HPLC and sensitively detected. The proposed method was successfully applied to the analyses of rat plasma and real products.

“指定物质”（Shitei-Yakubutsu）的结构分为三种类型，即，苯乙胺、色胺和胡椒碱。建立了高效液相色谱-多通道电化学检测器同时测定指定物质的方法。该方法利用在线氧化，是第一个同时测定多种指定物质的报告。建立了16种指定物质的超高效液相色谱-电喷雾飞行时间质谱联用检测方法。但是，荧光检测法测定几种指定物质时受到内源性物质的干扰。然而，在真实的样品中，通过UPLC分离和TOF-MS检测的组合成功地进行了测定。该方法已用于人血浆、尿液和真实的产品的分析。此外，以R（-）-DBD-PyNCS为手性试剂，采用间接手性衍生化方法实现了结构中碳原子不对称的苯乙胺类化合物的对映体拆分。采用反相高效液相色谱法对12种苯乙胺衍生物进行了有效分离和灵敏检测。该方法已成功地应用于大鼠血浆和真实的产品的分析。

项目成果

HPLC enantioseparation of α,α-diphenyl-2-pyrrolidinemethanol and methylphenidate using a chiral fluorescent derivatization reagent…

使用手性荧光衍生试剂对 α,α-二苯基-2-吡咯烷甲醇和哌醋甲酯进行 HPLC 对映分离……

• 发表时间: 2010
• 期刊: Journal of Separation Science
• 影响因子: 3.1
• 作者: Shinsuke Inagaki;et al.
• 通讯作者: et al.

Rapid detection of ketamine and norketamine in rat hair using micropulverized extraction and ultra-performance liquid chromatography-electrospray ionization mass spectrometry.

• DOI: 10.1002/bmc.1256
• 发表时间: 2009-12
• 期刊: Biomedical chromatography : BMC
• 作者: Shinsuke Inagaki;Hatsune Makino;T. Fukushima;J. Min;T. Toyo′oka

合田幸広:Diphenyl-2-pyrrolidine methanolおよびmethylphenidateの光学異性体分離

Yukihiro Goda：二苯基-2-吡咯烷甲醇和哌甲酯的光学异构体分离

• 发表时间: 2010
• 作者: 平島晴生;谷口さゆり;稲垣真輔;東達也;閔俊哲;豊岡利正;花尻瑠璃
• 通讯作者: 花尻瑠璃

合田幸広:HPLC-電気化学検出法を用いたデザイナードラッグの簡便かつ高感度な分析法の開発

Yukihiro Goda：使用 HPLC-电化学检测方法开发简单且高灵敏度的设计药物分析方法

• 发表时间: 2009
• 作者: 山下和秀;閔俊哲;豊岡利正;稲垣真輔;東達也;花尻瑠理
• 通讯作者: 花尻瑠理

HPLC enantioseparation of α,α-diphenyl-2-pyrrolidinemethanol and methylphenidate using a chiral fluorescent derivatization reagent and its application to the analysis of rat plasma.

使用手性荧光衍生试剂对 α,α-二苯基-2-吡咯烷甲醇和哌醋甲酯进行 HPLC 对映分离及其在大鼠血浆分析中的应用。

• DOI: 10.1002/jssc.201000479
• 发表时间: 2010
• 期刊: Journal of separation science
• 影响因子: 3.1
• 作者: Shinsuke Inagaki;Sayuri Taniguchi;Haruo Hirashima;T. Higashi;J. Min;R. Kikura;Y. Goda;T. Toyo′oka
• 通讯作者: T. Toyo′oka