Physiological significance of synaptotagmin-SNAP25 binding in neurotransmitter release

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To understand mechanisms of neurotransmitter release, the binding of synaptotagmin (syt) to SNARE complexes (SNAP-25-synatxin-synaptobrevin) was studied. Surprisingly, whereas SNAP-25 did not bind syt, which differs from previous findings, syntaxin bound syt in the absence of calcium ion (Ca^<2+>). Point mutagenesis and liposome fusion assay provide biochemical evidence that syt binds SNARE complexes via syntaxin before Ca2+ influx into presynaptic nerve terminals.