Studies on the roles for 8-nitroguanosine 3',5'-cyclic monophosphate in the cardiovascular system and the applications to drug discovery.

8-硝基鸟苷3,5-环单磷酸在心血管系统中的作用及其在药物发现中的应用研究。

• 批准号: 20590255
• 负责人: TOKUTOMI Yoshiko
• 金额: $ 3万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590255/
• 关键词: cGMP; eNOS; 心血管系; NO; 一酸化窒素; db

8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP), formed nitric oxide (NO)-dependently, is a novel physiological second messenger. To study the physiological activity of 8-nitro-cGMP in the cardiovascular system, we studied its effects on vascular reactivity of mouse aortas. 8-Nitro-cGMP induced enhancement of contraction to phenylephrine and relaxation concentration-dependently in aortas from non-diabetic mice, but only relaxation in aortas from diabetic mice. It was suggested that 8-nitrocGMP- induced superoxide production via eNOS uncoupling may mediate the enhancement of the phenylephrine contraction. The vasodilator effect of 8-nitro-cGMP may contribute to amelioration of the vascular endothelial dysfunction in diabetic mice, representing a novel pharmacological approach toprevent the complications associated with diabetes.

8-硝基鸟苷3 '，5'-环磷酸（8-nitro-cGMP）是一种新的生理第二信使，依赖一氧化氮（NO）形成。为了研究8-硝基环鸟苷酸在心血管系统中的生理活性，我们研究了其对小鼠血管反应性的影响。8-Nitro-cGMP浓度依赖性地增强非糖尿病小鼠平滑肌对苯丙氨酸的收缩和舒张作用，而仅增强糖尿病小鼠平滑肌对苯丙氨酸的舒张作用。提示8-nitrocGMP通过eNOS解偶联诱导的超氧化物产生可能介导了苯肾上腺素收缩的增强。8-nitro-cGMP的扩血管作用可能有助于改善糖尿病小鼠的血管内皮功能障碍，为预防糖尿病并发症提供了一种新的药理学途径。

项目成果

Effects of 8-nitro-cGMP on vascular responsiveness.

8-硝基-cGMP 对血管反应性的影响。

• 发表时间: 2009
• 期刊: Journal of Pharmacological Sciences 109(Suppl)
• 作者: Tokutomi Y;et al
• 通讯作者: et al

Critical Role of Apoptosis Signal-Regulating Kinase 1 in Aldosterone/Salt-Induced Cardiac Inflammation and Fibrosis

• DOI: 10.1161/hypertensionaha.109.135392
• 发表时间: 2009-09-01
• 期刊: HYPERTENSION
• 影响因子: 8.3
• 作者: Nakamura, Taishi;Kataoka, Keiichiro;Kim-Mitsuyama, Shokei
• 通讯作者: Kim-Mitsuyama, Shokei

8-Nitro-cGMPの血管系への作用

8-硝基-cGMP 对血管系统的影响

• 发表时间: 2009
• 作者: 松田直之;服部裕一;児玉亨;徳冨芳子
• 通讯作者: 徳冨芳子

Excess Salt Causes Cerebral Neuronal Apoptosis and Inflammation in Stroke-Prone Hypertensive Rats Through Angiotensin II-Induced NADPH Oxidase Activation

• DOI: 10.1161/strokeaha.108.517284
• 发表时间: 2008-11-01
• 期刊: STROKE
• 影响因子: 8.3
• 作者: Yamamoto, Eiichiro;Tamamaki, Nobuaki;Kim-Mitsuyama, Shokei
• 通讯作者: Kim-Mitsuyama, Shokei

Vascular responses to 8-nitro-cyclic GMP in non-diabetic and diabetic mice

非糖尿病和糖尿病小鼠对 8-硝基环 GMP 的血管反应

• DOI: 10.1111/j.1476-5381.2011.01201.x
• 发表时间: 2011
• 期刊: Br J Pharmabol
• 作者: Tokutomi Y.;et al
• 通讯作者: et al