Molecular mechanism of abnormal pH-homeostasis in the lumen of acidic organele from DjA2 knock out mice

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The DjA2 knockout mice showed lethal respiratory failure after birth. This was caused by insufficient alveolar expansion and the type 2 pneumocytes from the knockout mice had abnormal acidity in the lumen of lysosomal acidic organelle. To elucidate the molecular mechanism of maintaining homeostasis in the acidic organelle, I analyzed the pnumocytes by a physiological approach using ratiometry, the DjA2 by a molecular interaction study using Biacore, and the lung tissue by a proteomics approach.