Establishment of model system including human ABCC11 transgene

人类耳垢是一种由耵聍顶泌腺分泌的物质，分为湿型和干型。先前的分析表明SNP(c。ABCC11基因中的538G> A, rs17822931)是耳垢型决定因素。体臭有时是人类的神经问题之一。然而，这种气味的化学成分还不清楚。人体气味主要由携带G等位基因的人的大汗腺排出。奇怪的是，啮齿类动物基因组中缺失了完整的Abcc11基因，这可能是进化上的优势，可以通过没有体味来逃避敌人。因此，小鼠是一种天然的干型耳垢系统模型。先前的分析证实了ABCC11的另一个多态性（Δ27）。作为第一个项目，我们从日本发现了新的两个人withΔ27。一个是纯合子ofΔ27与AA，另一个是杂合子withΔ27与GA。第二个项目是乳腺癌与ABCC11多态性的关系。干燥型可能与雌激素受体阴性乳腺癌的发病率降低有关。我们之前发现了一种可以减少体味的补充剂。然而，尚未进行科学评价。作为第三个项目，我们分析了补品对G型耳垢人群腋臭的影响。我们发现在服用补充剂的人和没有服用补充剂的人之间有显著的差异。作为上一个项目，我们感兴趣的是小鼠是否有湿型ABCC11，分泌物质是如何增加或新排出的。含有ABCC11的小鼠细胞也可能对耐药性的研究有用。我们构建了包含ABCC11人类基因组DNA的转基因。然而，在小鼠胚胎干细胞中从未观察到足够的表达。现在我们正在进行ABCC11功能分析模型系统的生成。

Human earwax is a secreted material from ceruminous apocrine glands, consisting of wet and dry types. Previous analysis demonstrated a SNP(c. 538G> A, rs17822931) in the ABCC11 gene is the earwax-type determinant. Body odor is sometimes one of the nervous matters for human. However, the chemical constitution for the odor was not yet clear. The human body odor is mainly discharged from apocrine glands of persons with G allele.Curiously, rodent has genomic deletion of the full Abcc11 gene that might be evolutionally advantage to escape from enemy by means of no body odor. Thus, the mouse is a natural model system as dry type of ear wax.Previous analysis demonstrated another polymorphism(Δ27) of the ABCC11.As the first project, we detected new two people withΔ27 from Japanese. One is a homozygote ofΔ27 and AA, the other is a heterozygote withΔ27 and GA. The second project was how association of breast cancer with the ABCC11 polymorphism. It was possible that the dry type could be associated with reduction of frequency in the estrogen receptor negative breast cancer. We have previously found a supplement which might reduce the body odor. However, scientific evaluation had not yet performed. As the third project, we analyzed the effect of the supplement against the human axillary odor for the people with ear wax G type. We detected significant difference between the people who took the supplement against the no supplement. As the last project, we have interested if the mouse has the wet type ABCC11, how the secreted material was increased or newly discharged. The mouse cell including the ABCC11 might also useful for a study of drug resistance. We constructed the transgene including the human genomic DNA of the ABCC11.However, the adequate expression was never observed in the mouse ES cells. Now we are ongoing to generate the model system for analysis the function of the ABCC11.