Structural analysis of costimulatory molecules expressed on human γδ T cells

人γδ T细胞表达的共刺激分子的结构分析

• 批准号: 20590489
• 负责人: TANAKA Yoshimasa
• 金额: $ 3.08万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590489/
• 关键词: PD-1; PD-L1; 抗腫瘍効果; T細胞; T細胞受容体; 非ペプチド性抗原; テトラマー; IRp60; X線結晶解析; BirA; ストレプトアビジン; 免疫; カラムクロマトグラフィー; 結晶; タンパク質; 大腸菌; 二次構造; モノクローナル抗体; 抗原

The extracellular domains of human PD-1 and PD-L1 molecules were expressed in E. coli as inclusion bodies and refolded. The refolded proteins were purified on anion-exchange column chromatography and gel filtration. IRp60, which is expressed on human γδ T cells, was similarly expressed and purified, then tetramerized. The tetramer revealed that the ligand of IRp60 was expressed on antigen-presenting cells.

人PD-1和PD-L1分子的胞外结构域在大肠杆菌中以包涵体形式表达并折叠。经阴离子交换柱层析和凝胶过滤纯化。在人γδ T细胞上表达的IRp60同样被表达和纯化，然后四聚体化。四聚体显示IRp60配体在抗原呈递细胞上表达。

项目成果

Anti-PD-1 antibody reduces CD4+PD-1-T cells and relieves the lupus-like nephritis of NZB/W F1 mice

抗PD-1抗体减少CD4 PD-1-T细胞并缓解NZB/W F1小鼠的狼疮样肾炎

• 发表时间: 2010
• 期刊: The Journal of Immunology 184
• 作者: Kasagi;S.;et al.
• 通讯作者: et al.

Involvement of CD56brightCD11c+ cells in IL-18-mediated expansion of human γδ T cells

CD56brightCD11c+ 细胞参与 IL-18 介导的人 γδ T 细胞扩增

• 发表时间: 2011
• 期刊: J.Immunol 186
• 作者: Junko Tsuda;Wen Li;Hiromichi Yamanishi;Hideyuki Yamamoto;Akico Okuda;Shuji Kubo;Zhifeng Ma;Nobuyuki Terada;Yoshimasa Tanaka;Haruki Okamura.
• 通讯作者: Haruki Okamura.

Rapid γδ TCR-mediated responses in γδ T cells transduced with cancer-specific TCR genes

转导癌症特异性 TCR 基因的 γδ T 细胞中快速 γδ TCR 介导的反应

• 发表时间: 2009
• 期刊: Gene Therapy 16
• 作者: Atsunori Hiasa;Hiroyoshi Nishikawa;Michiko Hirayama;Shigehisa Kitano;Sachiko Okamoto;Hideto Chono;Seung Shin Yu;Junichi Mineno;Yoshimasa Tanaka;Nagahiro Minato;Ikunoshin Kato;Hiroshi Shiku.
• 通讯作者: Hiroshi Shiku.

ヒトγδ型T細胞を利用した新規がん標識免疫療法の確立

使用人类 γδ T 细胞建立新型癌症标记免疫疗法

• 发表时间: 2008
• 作者: 小林博人;田中義正;湊長博;田邉一成
• 通讯作者: 田邉一成

Complete remission of lung metastasis following adoptive immunotherapy using activated autologous gammadelta T-cells in a patient with renal cell carcinoma.

• 发表时间: 2010
• 期刊: Anticancer research
• 影响因子: 2
• 作者: Hirohito Kobayashi;Yoshimasa Tanaka;H. Shimmura;N. Minato;K. Tanabe