Measurement of pH in tumor tissue by 31P-MRS and microelectrode after glucose injection.

注射葡萄糖后用 31P-MRS 和微电极测量肿瘤组织的 pH 值。

• 批准号: 01570597
• 负责人: TANAKA Yoshimasa
• 金额: $ 1.54万
• 依托单位: KANSAI MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570597/
• 关键词: Hyperthermia; MRS; Glucose; pH; 熱感受性; グルコ-ス; 腫瘍内pH; 過血糖

Anti-tumor effects of hyperthermia are enhanced by lowering the pH in the tumor tissue with administration of glucose. This decreased pH in the tumor tissue with glucose administration was determined using a mouse experimental tumor. 31-MRSMicroelectrodes were used for the measurement of pH.By using these two measurement methods, time course change in the tumor tissue was determined in the controls and the groups treated with 6g/kg of intraperitoneal glucose. Following glucose admini13EA\ : stration, the tomor tissue showed a decrease of 0.3pH unit with the microelectrode method, but did not show any significant decrease in pH with the MRS determination. This finding suggested that 31-P-MRS.Showed intracellular pH(pHi) due to the localization of Pi and that the microelectrode indicated or extracellular pH(pHi). In spite of the decrease of 0.3pH unit in pHe, homogeneity of pHi was believed to be maintained owing to the buffer action of cells.

通过施用葡萄糖降低肿瘤组织中的pH来增强热疗的抗肿瘤作用。使用小鼠实验性肿瘤测定了给予葡萄糖后肿瘤组织中pH值的降低。31-采用MRS微电极测量pH值，采用这两种测量方法，测定对照组和腹腔注射6 g/kg葡萄糖组肿瘤组织的时程变化。用微电极法测定葡萄糖13 EA后肿瘤组织pH值下降0.3个单位，而用MRS法测定肿瘤组织pH值无明显下降。这一发现表明，31-P-MRS显示细胞内pH（pHi）是由于Pi的定位，而微电极指示细胞外pH（pHi）。尽管pHe降低了0.3pH单位，但由于细胞的缓冲作用，pHi的均一性被认为是保持的。

项目成果

北田憲彦: "マウス実験腫瘍における^<31>P-MRSを用いた温熱効果の評価-腫瘍組織中ATPと腫瘍成長曲線の対比-" 日本医学放射線学会雑誌. 52. 669-673 (1992)

Norihiko Kitada：“使用^ 31 P-MRS对实验小鼠肿瘤的热效应进行评估-肿瘤组织中的ATP和肿瘤生长曲线的比较-”日本医学放射学会杂志52. 669-673（1992）。

田中敬正: "温熱による生理学的変化-特に腫瘍の微小循環器系を中心に-" 消化器癌. 2. 227-232 (1992)

Takamasa Tanaka：“热引起的生理变化——尤其是肿瘤的微循环系统——”胃肠癌。2. 227-232 (1992)

永田憲司: "螢光pH指示薬を用いた細胞内pH測定と温熱による細胞内pHへの影響" CYTOMETRY RESEARCH. 2. 12-17 (1992)

Kenji Nagata：“使用荧光 pH 指示剂测量细胞内 pH 值以及热对细胞内 pH 值的影响”CYTOMETRY RESEARCH 2. 12-17 (1992)。

田中敬正: "組織環境状態と温熱感受性" CRC. 2. 18-25 (1993)

Takamasa Tanaka：“组织环境状态和热敏感性”CRC。2. 18-25 (1993)

田中敬正: "腫瘍の微小循環系に及ぼす温熱の影響とその修飾" Oncologia. 25. (445-449)57-61 (1992)

Takamasa Tanaka：“热对肿瘤微循环系统的影响及其修饰”Oncologia 25。(445-449)57-61 (1992)