Measurement of extracellular pH decrease cased by hyperthermiaand drugs on tumor cells.

测量热疗和药物对肿瘤细胞造成的细胞外 pH 值降低。

• 批准号: 04670682
• 负责人: TANAKA Yoshimasa
• 金额: $ 1.34万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670682/
• 关键词: Heat treatment; tumor cell; intracellular pH; Fluorecent dye; 螢光色素; 温熱耐性; 温熱療法; 温熱感受性; 膜作用物質

without affect of metabolic changes the authors measured intratumor pH by using 19-F magnetic resonance spectroscopy (MRS) with a fluorine compound (ZK-150471) on the basis of a curve by pH electrode.Using the 4.7 tesla magnetic resonance apparatus, a fluorine compound that had acid-base equilibrial change and was impermeable within cell membranes was used. The fluorine compound was injected intravenously. The signal was obtained from mouse mammary cancer by creating an experimental tumor on the leg of mice, And the tumors, which were heated with and without hydralazine. The pH evaluated from chemical shift of the fluorine compound. The pH data was obtained from an electrode for reference.The pH of nontreated tumors were 6.94. The p11 decreased to 6.722 at 20 minutes even after 20 minutes of heating.It is possible to measure the extracellular pH by 19F-MRS with the fluorine compound noninvasively in vivo, even after heating.

在不影响代谢变化的情况下，采用含氟化合物（ZK-150471）的19-F磁共振波谱仪（MRS）在pH电极曲线的基础上测定肿瘤内pH值。使用4.7特斯拉磁共振仪，使用一种具有酸碱平衡变化且在细胞膜内不渗透的氟化合物。氟化合物经静脉注射。该信号是通过在小鼠的腿上制造一个实验性肿瘤，从小鼠乳腺癌中获得的，并将肿瘤用和不含肼加热。由氟化合物的化学位移来计算pH值。从电极上获得pH值作为参考。未处理的肿瘤pH值为6.94。即使加热20分钟，p11在20分钟后也下降到6.722。即使在加热后，用氟化合物19F-MRS在体内无创测量细胞外pH值也是可能的。

项目成果

Yasushi Itagaki: "Rule of intracellular calcium concentration on tumor cell death from hyperthermia" Oncology Report. 5. 139-141 (1998)

Yasushi Itagaki：“细胞内钙浓度对肿瘤细胞因高温死亡的规则”肿瘤学报告。

Yoshizumi Aoki: "Measurement of intratumor pH by pH indicator used in 19F-magnetic resonance spectroscopy-Measurement of extra cellular pH decrease caused by hyperthermia conbined with hydralazin." InvestigaTive Radiology. 31. 680-689 (1996)

Yoshizumi Aoki：“通过 19F 磁共振波谱法中使用的 pH 指示剂测量肿瘤内 pH 值 - 测量与肼屈嗪相结合的热疗引起的细胞外 pH 值降低。”

Norihiko Kitada, Kiyoshi Akagi, Yoshimasa Tanaka, Bernnardd Fritz Zieroth.: "Evaluation of thermal damage hyperthermia on murine experimental tumor by 31P-NMR spectroscopy -Correlation between ATP and growth delays" J.of Radiat.Res. 138. 65-73 (1994)

Norihiko Kitada、Kiyoshi Akagi、Yoshimasa Tanaka、Bernnardd Fritz Zieroth.：“通过 31P-NMR 光谱评估小鼠实验肿瘤热损伤热损伤 - ATP 与生长延迟之间的相关性”J.of Radiat.Res。

Norihiko Kitada: "Evaluation of thermal damage hyperthermia on murine experimental tumor by 31P-NMR spectroscopy-Correlation between ATP and growth dalays." J of Radiat.Res.138. 65-73 (1994)

Norihiko Kitada：“通过 31P-NMR 光谱评估小鼠实验肿瘤的热损伤热疗 - ATP 与生长延迟之间的相关性。”

Takuro Nishida, Kiyoshi Akagi, Yoshimasa Tanaka: "Correlation between cell killing effect and cell membrane potential after heat treatment : analysis using fluorescent dye and flow cytometry" Int.J.Hyperthermia. 13. 227-234 (1997)

Takuro Nishida、Kiyoshi Akagi、Yoshimasa Tanaka：“热处理后细胞杀伤效果与细胞膜电位之间的相关性：使用荧光染料和流式细胞术进行分析”Int.J.Hyperthermia。