Measurement of extracellular pH decrease cased by hyperthermiaand drugs on tumor cells.

在不受代谢变化影响的情况下，作者使用氟化合物（ZK-150471），根据pH电极的曲线，使用4.7特斯拉磁共振装置，使用具有酸碱平衡变化且在细胞膜内不可渗透的氟化合物，通过19-F磁共振波谱（MRS）测量瘤内pH。氟化合物被静脉注射。这个信号是从小鼠乳腺癌中获得的通过在小鼠腿上制造一个实验性肿瘤，然后用肼苯哒嗪和不用肼苯哒嗪加热肿瘤。根据氟化合物的化学位移评价pH值。pH值数据由电极获得，未处理肿瘤的pH值为6.94。即使加热20分钟后，p11也在20分钟时下降到6.722，即使加热后，也可以在体内用氟化合物通过19 F-MRS非侵入性地测量细胞外pH。

without affect of metabolic changes the authors measured intratumor pH by using 19-F magnetic resonance spectroscopy (MRS) with a fluorine compound (ZK-150471) on the basis of a curve by pH electrode.Using the 4.7 tesla magnetic resonance apparatus, a fluorine compound that had acid-base equilibrial change and was impermeable within cell membranes was used. The fluorine compound was injected intravenously. The signal was obtained from mouse mammary cancer by creating an experimental tumor on the leg of mice, And the tumors, which were heated with and without hydralazine. The pH evaluated from chemical shift of the fluorine compound. The pH data was obtained from an electrode for reference.The pH of nontreated tumors were 6.94. The p11 decreased to 6.722 at 20 minutes even after 20 minutes of heating.It is possible to measure the extracellular pH by 19F-MRS with the fluorine compound noninvasively in vivo, even after heating.