Development of highly sensitive simultaneous bio- and chemi-luminescent enzyme immunoassay for periodontal pathogens genes

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We have developed simultaneous luminometric assay for aequorin, firefly luciferase and horseradish peroxidase. In this study, we applied this analysis method to the detection of the polymerase chain reaction (PCR) products. We selected the periodontal pathogens genes as a measurement genes. Among putative periodontal pathogens, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola are related most as etiological agents in periodontal disease. Therefore, detection of those three pathogens are useful to diagnosis for periodontal disease. We developed simultaneous detection of multiplex PCR products of three periodontal pathogens by using the bio- and chemi-luminescent enzyme immunoassay (BCLEIA). The simultaneous BCLEIA could be highly sensitive detection compared with conventional agarose gel electrophoresis.