Role of connexin-43 in protective signaling incardiomyocyte

Connexin-43 在心肌细胞保护性信号传导中的作用

• 批准号: 20590870
• 负责人: MIURA Tetsuji
• 金额: $ 3万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590870/
• 关键词: コネキシン; 細胞シグナル; 心筋細胞; 細胞死; connexin-43; signal transduction; G protein coupled receptor; β-subunit; mitochondria; 細胞内情報電達; 細胞内情報伝達

This study aimed to clarify role of connexin-43 (Cx43) in a major cytoprotective signaling, PI3K-Akt-GSK-3β signaling in cardiomyocytes. We found that Cx43 in the sarcolemma contributes to activation of class I_B P13K as a co-factor of β subunit of G proteins. This role of Cx43 is specific to regulation of class I_B P13K and is not required for activation of class I_A PI3K. Cx43 in mitochondria, being necessary for redox signaling by activated mitochondrial ATP-sensitive K^+ channel, was found to be dispensable in PI3K-Akt-GSK-3β signaling by G-protein coupled receptors.

本研究旨在阐明细胞间隙连接蛋白43(Cx43)在心肌细胞中主要的细胞保护信号PI3K-Akt-GSK-3β信号中的作用。我们发现肌膜中的Cx43作为G蛋白β亚单位的辅助因子参与了I_B类P13K的激活。Cx43的这种作用是针对I_B类P13K的调节，而不是激活I_A类PI3K所必需的。线粒体Cx43在G蛋白偶联受体介导的PI3K-Akt-GSK-3β信号转导中起着不可或缺的作用，是线粒体氧化还原信号传递所必需的。

项目成果

Connexin43-dependent mitochondrial GSK-3β phosphorylation in protection of cardiomyocyte against necrosis.

Connexin43 依赖性线粒体 GSK-3β 磷酸化保护心肌细胞免于坏死。

• 发表时间: 2010
• 作者: Ishikawa S;Miki T;Tanno M;Miura T.
• 通讯作者: Miura T.

Enhanced ANT-cyclophilin-D interaction underlies reduced anti-infarct tolerance and loss of protective response to erythropoietin in hypertensive hypertrophied hearts.

增强的 ANT-亲环蛋白-D 相互作用是高血压肥厚心脏中抗梗塞耐受性降低和对促红细胞生成素保护反应丧失的基础。

• 发表时间: 2009
• 作者: Miki T. Yano T;Miura T;Tanno M. Shimamoto K.
• 通讯作者: Tanno M. Shimamoto K.

Angiotensin II Type 1 Receptor-Mediated Upregulation of Calcineurin Activity Underlies Impairment of Cardioprotective Signaling in Diabetic Hearts

• DOI: 10.1161/circresaha.109.205385
• 发表时间: 2010-01-08
• 期刊: CIRCULATION RESEARCH
• 影响因子: 20.1
• 作者: Hotta, Hiroyuki;Miura, Tetsuji;Shimamoto, Kazuaki
• 通讯作者: Shimamoto, Kazuaki

Epicardium and pericardium: a joint force for infarct repair?

心外膜和心包：梗塞修复的合力？

• DOI: 10.1016/j.yjmcc.2010.01.012
• 发表时间: 2010
• 期刊: Journal of molecular and cellular cardiology
• 影响因子: 5
• 作者: T. Miura

Glycogen synthase kinase-3 inactivation is not required for ischemic preconditioning or postconditioning in the mouse

• DOI: 10.1161/circresaha.107.169953
• 发表时间: 2008-08-01
• 期刊: CIRCULATION RESEARCH
• 影响因子: 20.1
• 作者: Nishino, Yasuhiro;Webb, Ian G.;Marber, Michael S.
• 通讯作者: Marber, Michael S.