Prevention of diabetic nephropathy by MafB

我们研究了MafB在肾小球上皮细胞中的功能。通过数据库监测，我们在nephrin和podocin基因调控区发现了Maf识别元件（MARE）。凝胶迁移率变动分析鉴定MafB结合nephrin和podocin基因的MARE。报告基因检测也表明MafB可以通过MARE激活nephrin基因的转录。糖尿病肾病模型鼠肾组织中MafB的表达降低，nephrin的表达降低。MafB在肾小球上皮细胞中的过表达可预防糖尿病肾病。

We have investigated the function of MafB in glomerular epithelial cells. By database surveillance, we have found Maf recognition element (MARE) in the regulatory region of nephrin and podocin gene. Gel mobility shift assays identified that MafB binds to the MARE of the nephrin and podocin gene. Reporter assay also showed MafB could activate the nephrin gene transcription through the MARE. The expression of MafB was decreased in murine diabetic nephropathy models, as well as the expression of nephrin. Diabetic nephropathy might be prevented by overexpression of MafB in glomerular epithelial cells.