Functional analysis of kinetochore proteins by utilizing engineered fission yeast cells harboring a single chromosome.

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Kinetochore is a multi-protein complex essential for chromosome segregation. In this research, we constructed "single-chromosome fission yeast", in which all the genomic information is put together on one chromosome. By utilizing this yeast strain, we found that mitotic kinetochore take a special architecture, in which the central domain of the centromere protrudes from the both sides of the "core" consisting of the heterochromatin. We speculate that this special architecture is vital for equal separation of replicated chromosomes.