Meiotic transition of germline stem cells and molecular regulation of homologous chromosome recombination

生殖干细胞减数分裂转变与同源染色体重组的分子调控

• 批准号: 20770177
• 负责人: CHUMA Shinichiro
• 金额: $ 2.75万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770177/
• 关键词: 生殖細胞; 減数分裂; 発生; ゲノム; 相同組換え

In this research project, we developed a novel culture system in which an established germline stem cell line derived from mouse spermatogonia can be synchronously induced to enter into meiotic prophase in vitro. This culture system enabled detailed molecular and biochemical analyses on mammalian meiosis such as inductive and inhibitory signaling pathways, their crosstalk and chromatin dynamics during homologous chromosome recombination.

在本研究项目中，我们开发了一种新的培养体系，在该体系中，建立的小鼠精原细胞生殖系干细胞系可以在体外同步诱导进入减数分裂前期。该培养系统能够对哺乳动物减数分裂进行详细的分子和生化分析，如诱导和抑制信号通路、它们的串扰和同源染色体重组过程中的染色质动态。

项目成果

Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin

小鼠精原干细胞归巢至种系生态位取决于β1-整合素

• 发表时间: 2008
• 期刊: Cell Stem Cell. 3
• 作者: Kanatsu-Shinohara M;Takehashi M;Takashima S;Lee J;Morimoto H;Chuma S;Raducanu A;Nakatsuji N;Fassler R;Shinohara T
• 通讯作者: Shinohara T

Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile

• DOI: 10.1038/nsmb.1615
• 发表时间: 2009-06-01
• 期刊: NATURE STRUCTURAL & MOLECULAR BIOLOGY
• 影响因子: 16.8
• 作者: Reuter, Michael;Chuma, Shinichiro;Pillai, Ramesh S.
• 通讯作者: Pillai, Ramesh S.

Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline.

精子发生的超微结构特征及其在种系中的进化保守性。

• 发表时间: 2008
• 作者: 田村宏治;米井（田村）小百合;矢野十織;横山仁;井出宏之;Shinichiro Chuma
• 通讯作者: Shinichiro Chuma

Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline: Germinal granules in mammals

• DOI: 10.1016/j.mce.2008.11.009
• 发表时间: 2009-07-10
• 期刊: MOLECULAR AND CELLULAR ENDOCRINOLOGY
• 影响因子: 4.1
• 作者: Chuma, Shinichiro;Hosokawa, Mihoko;Nakatsuji, Norio
• 通讯作者: Nakatsuji, Norio

Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline : Germinal granules in

精子发生的超微结构特征及其在生殖系中的进化保守性：生殖颗粒

• 发表时间: 2008
• 期刊: Mol Cell Endocrinol
• 影响因子: 4.1
• 作者: Chuma;S et.al.
• 通讯作者: S et.al.