Small RNA and epigenetic control of the tudor-piwi pathway in the germline

种系中 tudor-piwi 通路的小 RNA 和表观遗传控制

• 批准号: 22770210
• 负责人: CHUMA Shinichiro
• 金额: $ 2.75万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770210/
• 关键词: 生殖細胞; ゲノム; RNA; 発生; トランスポゾン; トランスポ

The piwi-tudor pathway is essential for piRNA biogenesis and it acts to suppress retrotransposons in the germline. In this study, we carried out mass spec and high throughput sequencing analyses of the piwi-tudor complex and identified novel factors that function to control retrotransposons in germline stem cells at RNA and epigenetic levels. Genetic and biochemical analyses of a mutant of a mouse piwi revealed(1) an essential and physiological role of the piwi slicer activity in the germline and(2) a complementarity dependent rule required for the slicer activity mediated piRNA biogenesis. In a collaborative work, we characterized piwi pathway operation in an established cell line of spermatogonia and carried out multi-omics analyses. These results provide novel insights into key mechanisms that protect the germline genome from transposon activity.

piwi-tudor途径对于皮尔纳生物发生是必需的，并且其作用是抑制生殖系中的反转录转座子。在这项研究中，我们进行了质谱和高通量测序分析的piwi-tudor复合物，并确定了新的因素，功能控制生殖干细胞在RNA和表观遗传水平的反转录转座子。对小鼠piwi突变体的遗传和生物化学分析揭示了（1）piwi切割器活性在种系中的基本和生理作用和（2）切割器活性介导的皮尔纳生物发生所需的互补依赖性规则。在一项合作工作中，我们在一个已建立的精原细胞系中表征了piwi通路的运作，并进行了多组学分析。这些结果为保护生殖系基因组免受转座子活性影响的关键机制提供了新的见解。

项目成果

MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline.

• DOI: 10.1016/j.devcel.2011.01.005
• 发表时间: 2011-03-15
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Watanabe, Toshiaki;Chuma, Shinichiro;Yamamoto, Yasuhiro;Kuramochi-Miyagawa, Satomi;Totoki, Yasushi;Toyoda, Atsushi;Hoki, Yuko;Fujiyama, Asao;Shibata, Tatsuhiro;Sado, Takashi;Noce, Toshiaki;Nakano, Toru;Nakatsuji, Norio;Lin, Haifan;Sasaki, Hiroyuki
• 通讯作者: Sasaki, Hiroyuki

Germline tudor familygenes, retrotransposon silencing and spermatogenesis in mice

种系都铎家族基因、逆转录转座子沉默和小鼠精子发生

• 发表时间: 2011
• 作者: Kuramochi-Miyagawa S;et al;Shinichiro Chuma
• 通讯作者: Shinichiro Chuma

Mammalian tudorrelated genes in the male germline

雄性种系中的哺乳动物都铎相关基因

• 发表时间: 2011
• 作者: Kuramochi-Miyagawa S;et al;Shinichiro Chuma;Shinichiro Chuma.
• 通讯作者: Shinichiro Chuma.

Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing

• DOI: 10.1038/nature10672
• 发表时间: 2011-12-08
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Reuter, Michael;Berninger, Philipp;Pillai, Ramesh S.
• 通讯作者: Pillai, Ramesh S.

MVH in piRNA processing and gene silencing of retrotransposons

• DOI: 10.1101/gad.1902110
• 发表时间: 2010-05-01
• 期刊: GENES & DEVELOPMENT
• 影响因子: 10.5
• 作者: Kuramochi-Miyagawa, Satomi;Watanabe, Toshiaki;Nakano, Toru
• 通讯作者: Nakano, Toru