Detection of chromosomal mutation induced by heavy-ion beam irradiation using plant sex chromosome

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Mutations induced by the heavy-ion irradiation were analyzed by using the plant Y chromosome as a target. Five sex-changing mutants that have mutations on the Y chromosome were induced by gamma-ray, whereas fifteen sex-changing mutants were produced by heavy-ion irradiation. Mutated regions in these mutants were analyzed by using seventeen of Y-chromosome STS markers including newly developed ones. The result indicates that the mutations induced by heavy-ion irradiation tend to be smaller than that induced by gamma-ray irradiation.