Elucidation of the plant sex-determining gene using minute processing of the Y chromosome and expression profiling.

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Seventeen hermaphrodite mutants and ten asexual mutants that have deletions on the Y chromosome were produced by heavy-ion irradiation in the dioecious plant Silene latifolia. By performing next-generation sequencing, 1741 of Y-linked fragments were identified. The regions for sex determining genes were narrowed down by identifying commonly deleted fragment in hermaphrodite or asexual mutants, respectively. Expression-profiling method using laser microdissection was developed to determine genes expressing in the sex-specific tissues.