Development of precise re-examine system of newborn mass screening based on liquid chromatography-mass spectrometry (LC-MS)

新生儿群体筛查需要高灵敏度、高可靠性的分析方法。对于低出生体重儿，在第一次筛查中观察到较高的疾病假阳性率。在此背景下，我们建立了高效液相色谱-质谱联用技术（LC-MS）作为生物标志物的有效分析方法。此外，我们将温度响应色谱法应用于生物体液中麻醉剂的定量分析。在该色谱系统中，只使用水作为流动相，从绿色化学的角度来看，具有许多优点。

High-sensitive and reliable analytical methods are required for newborn mass screening. As for low-birth weight infants, high false positive rate of diseases are observed in the first screening assay. Based on this background, we developed the effective analytical methods of biomarker, utilizing the igh-performance liquid chromatography-mass spectrometry (LC-MS). Furthermore, we applied the temperature-responsive chromatography to quantification of anesthetic agents in biological fluids. In this chromatography system, only water is used as the mobile phase, and there are many advantages from the point of view of the green chemistry.