Development of HSCCC/MS for the efficient purification of natural actives.

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This study aims to develop useful purification utilized with mass spectrometric monitoring for high-speed counter-current chromatography (HSCCC), which is free of irreversible adsorption and offers high resolution comparable to column chromatography. HSCCC has been widely used in preparative separation and purification of natural, medical and biochemical products. Our study was reported that HSCCC system was efficiently applied to separation of the gentamicin and polypeptide using on-line mass spectrometric monitoring with electrospray ionization. This technique would be useful purification of bioactive compounds for continuous mass spectrometric data acquisition, and 'target'-guided fractionation of small/large molecular. In this report, the HSCCC coupled with mass spectrometry technique was developed for the purification of bioactive compounds in natural extracts.