Research on virulence regulatory mechanisms by CVF

CVF毒力调控机制研究

• 批准号: 20790057
• 负责人: KAITO Chikara
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790057/
• 关键词: 分子生物学; 病原性; 黄色プドウ球菌; RNA結合タンパク質; RNA分解酵素; カイコ感染モデル; 黄色ブドウ球菌; 病原性因子

We previously identified the cvfA gene (SA1129) that is required for the expression of the virulence regulatory locus agr in Staphylococcus aureus using silkworm infection model. CvfA cleaves 2', 3'-cyclic phosphodiester linkage at the 3'-teminal of RNA. In this study, we performed microarray analysis of the cvfA mutant to identify the genes whose expressions are under the control of the cvfA gene. In the cvfA-deletion mutant, the expression of 20% of total genes were increased or decreased over 2-fold compared with parent strain. The WH domain is critical to CvfB function and contains a unique sequence motif. Thus CvfB represents a novel assembly of modules for binding RNA.cvfC affects the expression of metabolic genes.

我们先前使用家蚕感染模型鉴定了金黄色葡萄球菌毒力调控位点agr表达所需的cvfA基因（SA1129）。CvfA在RNA的3‘端切割2’，3'-环磷酸二酯链。在这项研究中，我们对cvfA突变体进行了微阵列分析，以确定其表达受cvfA基因控制的基因。在cvfa -缺失突变株中，20%的总基因表达量比亲本株增加或减少2倍以上。WH结构域对CvfB功能至关重要，包含一个独特的序列基序。因此，CvfB代表了一种结合RNA的新型模块组装。cvfC影响代谢基因的表达。

项目成果

Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity.

• DOI: 10.1016/j.micpath.2008.10.010
• 发表时间: 2009-02
• 期刊: Microbial pathogenesis
• 影响因子: 3.8
• 作者: K. Usui;S. Miyazaki;C. Kaito;K. Sekimizu

A Novel Gene fudoh in SCCmec Region Regulates the Colony Spreading Ability and Virulence in Staphylococcus oureus

SCCmec 区域的新基因 fudoh 调节金黄色葡萄球菌的菌落传播能力和毒力

• 发表时间: 2008
• 作者: 内藤裕子;竹松弘;小堤保則;Kaito C
• 通讯作者: Kaito C

A Novel Gene, fudoh, in the SCCmec Region Suppresses the Colony Spreading Ability and Virulence of Staphylococcus aureus

• DOI: 10.1371/journal.pone.0003921
• 发表时间: 2008-12-11
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Kaito, Chikara;Omae, Yosuke;Sekimizu, Kazuhisa
• 通讯作者: Sekimizu, Kazuhisa

Phosphodiesterase activity of CvfA is required for virulence in Staphylococcus aureus

• DOI: 10.1074/jbc.m705309200
• 发表时间: 2008-01-25
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Nagata, Makiko;Kaito, Chikara;Sekimizu, Kazuhisa
• 通讯作者: Sekimizu, Kazuhisa

Gene Expression in Pre-MBT Embryos and Activation of Maternally-Inherited Program of Apoptosis to be Executed at around MBT as a Fail-Safe Mechanism in Xenopus Early Embryogenesis

MBT 前胚胎中的基因表达和在 MBT 前后执行的母系遗传细胞凋亡程序的激活作为非洲爪蟾早期胚胎发生的故障安全机制

• 发表时间: 2008
• 期刊: Gene Regulation and Systems Biology
• 作者: K. Shiokawa;M. Aso;T. Kondo;Hiroaki Uchiyama;S. Kuroyanagi;Jun;Senji Takahashi;M. Kajitani;C. Kaito;K. Sekimizu;E. Takayama;K. Igarashi;H. Hara
• 通讯作者: H. Hara