Research on virulence regulatory mechanisms by CVF

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We previously identified the cvfA gene (SA1129) that is required for the expression of the virulence regulatory locus agr in Staphylococcus aureus using silkworm infection model. CvfA cleaves 2', 3'-cyclic phosphodiester linkage at the 3'-teminal of RNA. In this study, we performed microarray analysis of the cvfA mutant to identify the genes whose expressions are under the control of the cvfA gene. In the cvfA-deletion mutant, the expression of 20% of total genes were increased or decreased over 2-fold compared with parent strain. The WH domain is critical to CvfB function and contains a unique sequence motif. Thus CvfB represents a novel assembly of modules for binding RNA.cvfC affects the expression of metabolic genes.