Development of simple mass screening method for the diagnosis of organic aciduria

建立了一种简便的用荧光法测定尿液中总二羧酸的方法筛选有机酸尿。这种方法是基于分子内形成的荧光衍生与芘试剂。这项技术是如此的选择性，它允许通过直接衍生化稀释的尿液样品的二羧酸总量的荧光测量。同样的反应混合物也作为高效液相色谱样品用于分离测定单个二羧酸。

A simple screening method of organic aciduria by fluorometric measurement of total dicarboxylic acids inhuman urine is developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent. This technique is so selective that it permits fluorometric measurement of total amount of dicarboxylic acids by the direct derivatization of diluted urine samples. The same reaction mixture was also served as a high-performance liquid chromatographic sample for the separative determination of individual dicarboxylic acids.