The nuclear medicine study of connexin43 expression and denervation in rabbit heart with myocardial infarction

心肌梗死兔心脏connexin43表达及去神经的核医学研究

• 批准号: 20790555
• 负责人: AMINO Mari
• 金额: $ 2.58万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790555/
• 关键词: 致死性心室性不整脈; コネキシン43; ウサギ梗塞モデル; 心臓核医学検査; 心室頻拍・心室細動; 心筋核医学検査; ウサギ心筋梗塞モデル; 交感神経リモデリング; 電気的リモデリング

Fatal arrhythmia such as ventricular tachycardia and ventricular fibrillation (VT/VF) occurs to a high rate for the condition of a patient with a myocardial infarction or heart failure. We examined the quantification of connexin 43 (Cx43) expressions by using the nuclear medicine techniques in the rabbit infarction model, and investigated the relation between the abnormal Cx43 distribution and the change of the norepinephrine metabolism of the sympathetic nerve ending (functional denervation) using 123I-metaiodobenzyleguanidine (MIBG) nuclear medicine. In the results of ^<123>I-anti-Cx43 in vitro ARG, the accumulation of Cx43 decreased at the infarction and neighboring areas which were corresponded to be confirmed by the Cx43 immunostaining, Azan stain, and MIBG exam. In addition the accumulation of Cx43 decreased in the interventricular septum. Because there were bundle of His and Purkinje fibers in the interventricular septum, and this mainstream gap junction was Cx40, therefore the decreased expressions of Cx43 might reflect the relative attenuation. The ^<123>I-anti-Cx43 in vitro ARG has a possibility to be effective for an identification of the diseased and physiological distribution of Cx43.

致命性心律失常，如室性心动过速和心室纤颤（VT/VF），在心肌梗死或心力衰竭患者的情况下发生率很高。应用核医学技术检测了家兔脑梗死模型中连接蛋白43（Cx43）的定量表达，并应用123 I-间碘苄胍（MIBG）核医学技术研究了Cx43的异常分布与交感神经末梢去甲肾上腺素代谢变化的关系。在~（13）<123>I-抗-Cx43体外ARG实验结果中，Cx43在梗死区及邻近区域的聚集减少，与Cx43免疫染色、Azan染色及MIBG检测结果一致，此外，Cx43在室间隔的聚集也减少。由于室间隔内存在希氏纤维束和浦肯野纤维束，而这一主流缝隙连接为Cx40，因此Cx43表达的降低可能反映了相对衰减。抗<123>Cx43的体外ARG有可能有效地用于鉴定Cx43的患病和生理分布。

项目成果

イヌ心筋梗塞モデルに対する重粒子線単回照射による心室内遅延電位の長期的改善効果

重离子束单次照射对犬心肌梗死模型脑室内延迟电位的远期改善作用

• 发表时间: 2008
• 作者: 網野真理;他
• 通讯作者: 他

Single Heavy Ion Irradiation to Rabbit Hearts Maintains the Antiarryhthmic Effects due to Upregulation of Connexin 43 for One Year

对兔心脏进行单次重离子照射，由于连接蛋白 43 上调而维持一年的抗心律失常作用

• 发表时间: 2008
• 作者: Mari Amino;et.al.
• 通讯作者: et.al.

Year-long upregulation of connexin43 in rabbit hearts by heavy ion irradiation

• DOI: 10.1152/ajpheart.00160.2009
• 发表时间: 2010-03-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
• 影响因子: 4.8
• 作者: Amino, Mari;Yoshioka, Koichiro;Tanabe, Teruhisa
• 通讯作者: Tanabe, Teruhisa

重粒子線単回照射がウサギ心筋のコネキシン43発現に及ぼす長期的効果の検討

单剂量重离子束照射对兔心肌连接蛋白43表达的长期影响研究

• 发表时间: 2010
• 作者: 網野;ら
• 通讯作者: ら

Upregulation of Connexin43 in Rabbit Hearts by Single Heavy Ion Irradiation Lasts over One Year

单次重离子照射对兔心脏 Connexin43 的上调持续一年多

• 发表时间: 2009
• 作者: Mari Amino;et.al.
• 通讯作者: et.al.