The nuclear medicine study of connexin43 expression and denervation in rabbit heart with myocardial infarction

致命性心律失常，如室性心动过速和心室纤颤（VT/VF），在心肌梗死或心力衰竭患者的情况下发生率很高。应用核医学技术检测了家兔脑梗死模型中连接蛋白43（Cx43）的定量表达，并应用123 I-间碘苄胍（MIBG）核医学技术研究了Cx43的异常分布与交感神经末梢去甲肾上腺素代谢变化的关系。在~（13）<123>I-抗-Cx43体外ARG实验结果中，Cx43在梗死区及邻近区域的聚集减少，与Cx43免疫染色、Azan染色及MIBG检测结果一致，此外，Cx43在室间隔的聚集也减少。由于室间隔内存在希氏纤维束和浦肯野纤维束，而这一主流缝隙连接为Cx40，因此Cx43表达的降低可能反映了相对衰减。抗<123>Cx43的体外ARG有可能有效地用于鉴定Cx43的患病和生理分布。

Fatal arrhythmia such as ventricular tachycardia and ventricular fibrillation (VT/VF) occurs to a high rate for the condition of a patient with a myocardial infarction or heart failure. We examined the quantification of connexin 43 (Cx43) expressions by using the nuclear medicine techniques in the rabbit infarction model, and investigated the relation between the abnormal Cx43 distribution and the change of the norepinephrine metabolism of the sympathetic nerve ending (functional denervation) using 123I-metaiodobenzyleguanidine (MIBG) nuclear medicine. In the results of ^<123>I-anti-Cx43 in vitro ARG, the accumulation of Cx43 decreased at the infarction and neighboring areas which were corresponded to be confirmed by the Cx43 immunostaining, Azan stain, and MIBG exam. In addition the accumulation of Cx43 decreased in the interventricular septum. Because there were bundle of His and Purkinje fibers in the interventricular septum, and this mainstream gap junction was Cx40, therefore the decreased expressions of Cx43 might reflect the relative attenuation. The ^<123>I-anti-Cx43 in vitro ARG has a possibility to be effective for an identification of the diseased and physiological distribution of Cx43.