The nuclear medicine study of connexin43 expression and denervation in rabbit heart with myocardial infarction

心肌梗死兔心脏connexin43表达及去神经的核医学研究

• 批准号: 20790555
• 负责人: AMINO Mari
• 金额: $ 2.58万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790555/
• 关键词: 致死性心室性不整脈; コネキシン43; ウサギ梗塞モデル; 心臓核医学検査; 心室頻拍・心室細動; 心筋核医学検査; ウサギ心筋梗塞モデル; 交感神経リモデリング; 電気的リモデリング

Fatal arrhythmia such as ventricular tachycardia and ventricular fibrillation (VT/VF) occurs to a high rate for the condition of a patient with a myocardial infarction or heart failure. We examined the quantification of connexin 43 (Cx43) expressions by using the nuclear medicine techniques in the rabbit infarction model, and investigated the relation between the abnormal Cx43 distribution and the change of the norepinephrine metabolism of the sympathetic nerve ending (functional denervation) using 123I-metaiodobenzyleguanidine (MIBG) nuclear medicine. In the results of ^<123>I-anti-Cx43 in vitro ARG, the accumulation of Cx43 decreased at the infarction and neighboring areas which were corresponded to be confirmed by the Cx43 immunostaining, Azan stain, and MIBG exam. In addition the accumulation of Cx43 decreased in the interventricular septum. Because there were bundle of His and Purkinje fibers in the interventricular septum, and this mainstream gap junction was Cx40, therefore the decreased expressions of Cx43 might reflect the relative attenuation. The ^<123>I-anti-Cx43 in vitro ARG has a possibility to be effective for an identification of the diseased and physiological distribution of Cx43.

致命的心律不齐，例如心室心动过速和心室纤颤（VT/VF），患有心肌梗塞或心脏衰竭的患者的状况很高。我们通过使用兔梗死模型中的核医学技术研究了连接蛋白43（CX43）表达式的定量，并研究了使用123i-Metaiodobenzyidobenzyideinine（MitebibgaNIDININININE（Mibibenide）（Mibenice）。在体外ARG的 ^<123> i-anti-cx43的结果中，在梗塞和邻近区域的CX43的积累降低了，这些区域通过CX43免疫染色，AZAN染色和MIBG检查证实。另外，在介入隔膜中，CX43的积累减少。因为室内隔膜中有他的和普肯野纤维的束，并且这种主流间隙连接为CX40，因此CX43的表达降低可能反映了相对衰减。 ^<123> i-anti-cx43体外ARG有可能有效鉴定CX43的患病和生理分布。

项目成果

Upregulation of Connexin43 in Rabbit Hearts by Single Heavy Ion Irradiation Lasts over One Year

单次重离子照射对兔心脏 Connexin43 的上调持续一年多

• 发表时间: 2009
• 作者: Mari Amino;et.al.
• 通讯作者: et.al.

イヌ心筋梗塞モデルに対する重粒子線単回照射による心室内遅延電位の長期的改善効果

重离子束单次照射对犬心肌梗死模型脑室内延迟电位的远期改善作用

• 发表时间: 2008
• 作者: 網野真理;他
• 通讯作者: 他

Single Heavy Ion Irradiation to Rabbit Hearts Maintains the Antiarryhthmic Effects due to Upregulation of Connexin 43 for One Year

对兔心脏进行单次重离子照射，由于连接蛋白 43 上调而维持一年的抗心律失常作用

• 发表时间: 2008
• 作者: Mari Amino;et.al.
• 通讯作者: et.al.

重粒子線単回照射がウサギ心筋のコネキシン43発現に及ぼす長期的効果の検討

单剂量重离子束照射对兔心肌连接蛋白43表达的长期影响研究

• 发表时间: 2010
• 作者: 網野;ら
• 通讯作者: ら