Development of new treatment for fatal ventricular tachyarrhythmias using heavy ion irradiation

利用重离子照射开发致命性室性快速心律失常的新疗法

• 批准号: 23790880
• 负责人: AMINO Mari
• 金额: $ 2.66万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790880/
• 关键词: コネキシン43; 重粒子線照射; 心室遅延電位; 心室遅延電; コネキシン; 心機能; 心筋梗塞モデル; 致死性心室性不整脈

The aim of this study is to investigate longitudinal changes in electromechanical performance of myocardial infarction (MI) dog hearts by targeted heavy ion irradiation (THIR). Four dogs were treated with THIR (12C6r, 15Gy; RIKEN,Japan) 2W after MI. Signal-averaged ECGs and echocardiograms were obtained longitudinally over 1 yr. Inducibility of fatal ventricular tachyarrhythmias (VT/VF) and connexin 43(Cx43) immunolabeling of the left ventricular tissues were studied 1 yr after MI. Immunolabeled Cx43 were significantly increased in dogs with THIR at non-ischemic zone and ischemic-border zone. Filtered-QRS increased 2W after MI and remained unchanged up to 1 yr in dogs without THIR, while returned toward baseline in dogs with THIR. RMS40 reduced 2W after MI, and then returned toward baseline only in dogs with THIR. VT/VFs were induced in 1/4 with THIR, whereas in 4/4 without THIR. LV systolic function inhibited 2W after MI, however 1 yr recovery was significantly enhanced by THIR.

这项研究的目的是通过靶向重离子照射（THIR）研究心肌梗塞（MI）狗心脏机电性能的纵向变化。 Mi后2W用2W的thir治疗四只狗（12c6r，15gy； riken，日本）。在1年内纵向获得了信号平均的ECG和超声心动图。 MI后1年研究了1年后，研究了左心室组织的免疫标记的诱导性（VT/VF）和连接蛋白43（CX43）的免疫标记。在非缺血区域和缺血性区域的狗的狗中，免疫标记的CX43显着增加。 MI后过滤的QR增加了2W，并且在没有窃听的狗中保持不变，而狗则在有窃贼的狗中返回基线。 RMS40在MI后减少了2W，然后仅在有斜向的狗中返回基线。 VT/VF在1/4中用斜向诱导，而在4/4中无窃听。 LV收缩功能在MI后抑制了2W，但是THIR显着增强了1年的回收率。

项目成果

重粒子線照射が梗塞後の不整脈基質に及ぼす長期効果 : イヌ心筋梗塞モデルを用いた検討

重离子束照射对梗塞后心律失常基质的长期影响：使用犬心肌梗塞模型的研究

• 发表时间: 2012
• 作者: Suzuki S;Yoshihisa A;Sugimoto K;Yamaki T;Kunii H;Nakazato K;Suzuki H;Saitoh S;Takeishi Y;網野真理
• 通讯作者: 網野真理

不整脈学

心律失常学

• 发表时间: 2012
• 作者: 井上博;村川裕二 編
• 通讯作者: 村川裕二 編

Heavy ion irradiation after myocardial infarction facilitates long-term recovery of electromechanical synchrony of canine hearts in association with Cx43 upregulation

心肌梗死后重离子照射促进与 Cx43 上调相关的犬心脏机电同步的长期恢复

• 发表时间: 2013
• 作者: 宮田真希子;義久精臣;八巻尚洋;及川雅啓;竹石恭知;Mari Amino
• 通讯作者: Mari Amino