Relationship between administration of molecular hydrogen and stroke

分子氢给药与中风之间的关系

• 批准号: 20790628
• 负责人: NISHIYAMA Yasuhiro
• 金额: $ 1.25万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790628/
• 关键词: 脳卒中; 水素水; 水素ガス; 脳梗塞

Clinical evidence as well as experimental results strongly suggests the major contribution of oxidative stress to stroke. The recent study reported that molecular hydrogen (H2) reduces oxidative stress and limits infarct volume of the brain. Moreover, the other study showed that the consumption of water with dissolved H2 to a saturated level prevents stress nigrostriatal degeneration. A variety of H2 delivery systems are used for effective administration. I investigate whether this simple method to deliver H2 into body or organs has the potential to prevent stroke. I tried two preliminary experiments. First, a separate population of 8-week-old SHRSPs was divided into two groups of 18 animals each and one group administered H2 water, which is replaced each day. Mean survival day did not differ significantly between two groups (DW group 107.5±38.6 days vs H2 group 105.85±39.5 days). This study suggested that giving hydrogen water to SHRSP rats did not influence the survival time. Second, rats were subjected to transient focal ischemia for 90 min by the intraluminal suture technique. Rats received intravenous injections of 5.0mg/kg of hydrogen dissolved in saline water or saline. Hydrogen water degassed by gentle stirring was used for control animals. Rats were ramdomly divided into three groups of 5 animals each: (I) 5 min before reperfusion group; (II) 60 min after reperfusion group, (III) saline group (control). But there is no significant difference in the infarction volume among three groups. This study suggested that injection of hydrogen dissolved in saline may have the potential effect on ischemic stroke, but further study is required to investigate how the timing or the route of administration of H2 is appropriate.

临床证据以及实验结果强烈表明，氧化应激对中风的主要贡献。最近的研究报告，分子氢（H2）减少氧化应激和限制脑梗死体积。此外，另一项研究表明，溶解H2的水的消耗达到饱和水平可以防止应激黑质纹状体变性。各种H2递送系统用于有效施用。我调查这种将H2输送到身体或器官的简单方法是否有可能预防中风。我做了两个初步实验。首先，将8周龄SHRSP的单独群体分成两组，每组18只动物，一组给予每天更换的H2水。两组间平均生存时间无显著差异（DW组107.5±38.6天vs H2组105.85±39.5天）。本研究提示，给予SHRSP大鼠氢水不影响其存活时间。第二，大鼠进行短暂的局灶性缺血90分钟的管腔内缝合技术。大鼠静脉注射溶于盐水或生理盐水的5.0mg/kg氢。将通过温和搅拌脱气的氢水用于对照动物。将大鼠随机分为再灌注前5 min组、再灌注后60 min组和生理盐水对照组，每组5只。但三组间梗死体积无显著性差异。这项研究表明，注射溶于生理盐水的氢可能对缺血性中风有潜在的影响，但需要进一步的研究，以探讨如何适当的时间或途径的管理H2。