Relationship between administration of molecular hydrogen and stroke

临床证据和实验结果有力地表明了氧化应激对中风的主要贡献。最近的研究报道，分子氢(H2)可减少氧化应激，限制脑梗塞体积。此外，另一项研究表明，将溶解氢的水消耗到饱和水平，可以防止应激黑质纹状体退化。各种氢气输送系统被用于有效的给药。我调查这种将氢气输送到身体或器官中的简单方法是否具有预防中风的潜力。我尝试了两个初步实验。首先，8周大的SHRSP被分成两组，每组18只，一组接受每天更换的H2水。两组患者平均生存天数差异无统计学意义(DW组107.5±38.6d，H2组105.85±39.5d)。本研究提示SHRSP大鼠给予氢气水不影响存活时间。采用线栓法复制大鼠短暂性局灶性脑缺血90min模型。给大鼠静脉注射溶解于盐水或生理盐水中的氢气5.0 mg/kg。用温和搅拌脱气的氢水作为对照动物。将大鼠随机分为3组，每组5只：(1)再灌注前5min组，(2)再灌注60min组，(3)生理盐水组(对照组)。但三组间脑梗塞体积差异无统计学意义。本研究提示，注射溶于生理盐水的氢气可能对缺血性卒中有潜在作用，但氢气的给药时机或给药途径如何才是合适的，还需要进一步的研究。

Clinical evidence as well as experimental results strongly suggests the major contribution of oxidative stress to stroke. The recent study reported that molecular hydrogen (H2) reduces oxidative stress and limits infarct volume of the brain. Moreover, the other study showed that the consumption of water with dissolved H2 to a saturated level prevents stress nigrostriatal degeneration. A variety of H2 delivery systems are used for effective administration. I investigate whether this simple method to deliver H2 into body or organs has the potential to prevent stroke. I tried two preliminary experiments. First, a separate population of 8-week-old SHRSPs was divided into two groups of 18 animals each and one group administered H2 water, which is replaced each day. Mean survival day did not differ significantly between two groups (DW group 107.5±38.6 days vs H2 group 105.85±39.5 days). This study suggested that giving hydrogen water to SHRSP rats did not influence the survival time. Second, rats were subjected to transient focal ischemia for 90 min by the intraluminal suture technique. Rats received intravenous injections of 5.0mg/kg of hydrogen dissolved in saline water or saline. Hydrogen water degassed by gentle stirring was used for control animals. Rats were ramdomly divided into three groups of 5 animals each: (I) 5 min before reperfusion group; (II) 60 min after reperfusion group, (III) saline group (control). But there is no significant difference in the infarction volume among three groups. This study suggested that injection of hydrogen dissolved in saline may have the potential effect on ischemic stroke, but further study is required to investigate how the timing or the route of administration of H2 is appropriate.