Defective erythropoiesis and ribosomal protein genes : Analysis of zebrafish model for Diamond-Blackfan anemia

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To investigate the pathogenic mechanism of Diamond-Blackfan anemia (DBA), I have developed a zebrafish DBA model by knocking down the ribosomal protein s19 gene using morpholino antisense oligo. Translational efficiency or production of total proteins in this model seemed almost same as control embryos. However, they showed the defective maturation of erythroid lineage by 2-3 days post fertilization. This suggests that there is an unknown mechanism that affect translation of specific factors, especially those involved in the erythroid pathway.