Expressional control of p27Kip1 in neuronal progenitor cells in vivo

体内神经元祖细胞中 p27Kip1 的表达控制

• 批准号: 20790744
• 负责人: MITSUHASHI Takayuki
• 金额: $ 2.58万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790744/
• 关键词: 小児神経学; 神経前駆細胞; 大脳皮質発生; 分化誘導; 細胞周期; p27Kip1; マウス

p27^<Kip1>, cell cycle regulatory protein, play a critical role in differentiation of neuronal progenitor cells (NPCs). A purpose of this research was to indentify transcriptional factors regulating p27^<Kip1> expression in NPCs during neocortical development. In binding assay, a signal was detected by using biotinylated oligonucletides of p27^<Kip1> 5' UTR promoter sequence together with nuclear extract from NPCs, indicating that DNA binding factor(s) was bound to the probe. However, in the samples with anti-FOXO1a/3a antibodies, alteration of signal mobility was not observed. Taken together, DNA binding factor(s) is not identified as FOXO1a/3a as previously speculated.

p27 β<Kip1>是细胞周期调控蛋白，在神经前体细胞（NPCs）的分化过程中起关键作用。本研究的目的是鉴定<Kip1>在新皮层发育过程中调节NPC中p27 α表达的转录因子。在结合测定中，通过使用p27^ 5' UTR启动子序列的生物素化寡核苷酸<Kip1>以及来自NPC的核提取物检测信号，表明DNA结合因子与探针结合。然而，在具有抗FOXO 1a/3a抗体的样品中，未观察到信号迁移率的改变。综上所述，DNA结合因子未被鉴定为先前推测的FOXO 1a/3a。

项目成果

Cell cycle. In Encyclopedia of Neuroscience. Volume III, pp. 588-591. Binder MD, Hirokawa N, Windhorst U, Hirsch MC (eds), Murakami F (section ed).

细胞周期。

• 发表时间: 2009
• 作者: Mitsuhashi T;Takahashi T.
• 通讯作者: Takahashi T.

Developmental regulation of acetylation statuses of histone proteins in neural progenitor cells of murine cerebral wall

小鼠脑壁神经祖细胞组蛋白乙酰化状态的发育调控

• 发表时间: 2010
• 作者: Mitsuhashi T;Takahashi T.
• 通讯作者: Takahashi T.

Coordinated regulation between cell cycle kinetics of neural progenitor cells and layer-specific phenotype of neocortical projection neuron

神经祖细胞的细胞周期动力学与新皮质投射神经元的层特异性表型之间的协调调节

• 发表时间: 2008
• 作者: Mitsuhashi T;Goto T;Takahashi T.
• 通讯作者: Takahashi T.

Cell cycle. In Encyclipedic Reference of Neuroscience

细胞周期。

• 发表时间: 2009
• 作者: Mitsuhashi T;Takahashi T.;Yoshiyama M;Mitsuhashi T. Takahashi T.
• 通讯作者: Mitsuhashi T. Takahashi T.

Genetic regulation of proliferation/differentiation characteristics of neural progenitor cells in the developing neocortex

• DOI: 10.1016/j.braindev.2009.05.002
• 发表时间: 2009-08-01
• 期刊: BRAIN & DEVELOPMENT
• 影响因子: 1.7
• 作者: Mitsuhashi, Takayuki;Takahashi, Takao
• 通讯作者: Takahashi, Takao