Expressional control of p27Kip1 in neuronal progenitor cells in vivo

P27；&lt；Kip1&gt；是一种细胞周期调节蛋白，在神经前体细胞的分化过程中起着关键作用。这项研究的目的是确定在新皮质发育过程中调节鼻咽癌p27^&lt；Kip1和gt；表达的转录因子。在结合实验中，利用p27^&lt；Kip1&gt；5‘UTR启动子序列的生物素化寡核苷酸与鼻咽癌细胞核提取液检测到一个信号，表明DNA结合因子(S)与该探针结合。然而，在具有抗FOXO1a/3a抗体的样本中，没有观察到信号迁移率的变化。综上所述，DNA结合因子(S)并不像先前推测的那样是FOXO1a/3a。

p27^<Kip1>, cell cycle regulatory protein, play a critical role in differentiation of neuronal progenitor cells (NPCs). A purpose of this research was to indentify transcriptional factors regulating p27^<Kip1> expression in NPCs during neocortical development. In binding assay, a signal was detected by using biotinylated oligonucletides of p27^<Kip1> 5' UTR promoter sequence together with nuclear extract from NPCs, indicating that DNA binding factor(s) was bound to the probe. However, in the samples with anti-FOXO1a/3a antibodies, alteration of signal mobility was not observed. Taken together, DNA binding factor(s) is not identified as FOXO1a/3a as previously speculated.