The utility of glial molecular imaging in epileptic animal brain.

神经胶质分子成像在癫痫动物大脑中的应用。

• 批准号: 20790896
• 负责人: HOSOI Rie
• 金额: $ 2.5万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790896/
• 关键词: グリア; 酢酸; ケトン; リチウムピロカルピン; てんかん; げっ歯類; 脳; 代謝; 幼弱ラット; グリア代謝; モノカルボン酸; ^<14>C-酢酸; ^<14>C-乳酸; 解糖; ラット; 幼弱動物; ケトン体

Glial energy metabolism in rat brain was markedly enhanced during a prolonged seizure induced by lithium-pilocarpine (Li-P) model. Glial metabolism was measured with ^<14>C-acetate. The increase of brain uptake was observed in adult (8 weeks old) rat brain. ^<14>C-acetate uptake in young (3 weeks old) rat brain was not changed during status epilepticus (SE), whereas both local cerebral blood flow and glucose metabolism were increased in young rat brain during SE. The anticonvulsant effects of acetone have been reported in various animal models of epilepsy. We also demonstrated that ketone bodies, methyl ethyl ketone (MEK) and diethyl ketone (DEK), suppressed SE that was induced by Li-P in rat. And MEK and DEK showed broad-spectra anticonvulsant effects in both chemically- and electroshock-induced experimental seizure models.

在锂-匹罗卡品（Li-P）致痫大鼠模型中，胶质细胞能量代谢明显增强。神经胶质代谢用13 <14>C-乙酸盐测量。在成年（8周龄）大鼠脑中观察到脑摄取增加。在<14>癫痫持续状态（SE）期间，年轻大鼠（3周龄）脑中的^C-乙酸摄取没有变化，而在SE期间，年轻大鼠脑中的局部脑血流量和葡萄糖代谢均增加。丙酮的抗惊厥作用已在各种癫痫动物模型中报道。我们还证明了酮体，甲基乙基酮（MEK）和二乙基酮（DEK），抑制由Li-P诱导的大鼠SE。MEK和DEK在化学和电休克诱导的实验性癫痫模型中均显示出广谱抗惊厥作用。

项目成果

De-coupling of blood flow and metabolism in the rat brain induced by glutamate.

谷氨酸诱导大鼠脑内血流和代谢的解耦。

• 发表时间: 2009
• 期刊: Ann Nucl Med. 23(3)
• 作者: Hirose S;Momosaki S;Sasaki K;Hosoi R;Abe K;Gee A;Inoue O.
• 通讯作者: Inoue O.

イボテン酸注入早期のラット脳における糖代謝の亢進と酸素代謝について

早期注射鹅膏蕈酸后大鼠脑内糖代谢和氧代谢的增强

• 发表时间: 2008
• 作者: 細井理恵;桃崎壮太郎;合瀬恭幸;福田肇;樋掛正明;林拓也;渡部浩司;三宅義徳;飯田秀博;井上修
• 通讯作者: 井上修

Remarkable increase in 14C-acetate uptake in an epilepsy model rat brain induced by lithium-pilocarpine

锂-毛果芸香碱诱导癫痫模型大鼠大脑中 14C-乙酸盐的摄取显着增加

• 发表时间: 2010
• 期刊: Brain Res. 1311
• 作者: Hosoi R;Kitano D;Momosaki S;Kuse K;Gee A;Inoue O.
• 通讯作者: Inoue O.

Inhibitory effect of methyl ethyl ketone upon the enhancement of cerebral blood flow during status epilepticus induced by lithium-pilocarpine.

甲乙酮对锂毛果芸香碱诱发癫痫持续状态时脑血流增强的抑制作用。

• 发表时间: 2009
• 期刊: Neurosci Lett. 462(3)
• 作者: Sugiyama E;Matsuki Y;Momosaki S;Hosoi R;Hasebe N;Abe K;Inoue O.
• 通讯作者: Inoue O.

Glucose utilization in the brain during acute seizure is a useful biomarker for the evaluation of anticonvulsants : effect of methyl ethyl ketone in lithium-pilocar pine status epilepticus rats.

急性癫痫发作期间大脑中的葡萄糖利用是评估抗惊厥药的有用生物标志物：甲基乙基酮对锂-匹罗卡松癫痫持续状态大鼠的作用。

• 发表时间: 2009
• 期刊: Nucl Med Biol. 36
• 作者: Yamada A;Momosaki S;Hosoi R;Abe K;Yamaguchi M;Inoue O.
• 通讯作者: Inoue O.