Integrative genomic analysis and screening of microRNA expression status in endometrial cancer.

子宫内膜癌中 microRNA 表达状态的综合基因组分析和筛选。

• 批准号: 20791165
• 负责人: HIRASAWA Akira
• 金额: $ 2.66万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791165/
• 关键词: 子宮体癌; ゲノム異常; メチル化; マイクロRNA; がんゲノム

MicroRNAs (miRNAs) are small non-coding RNAs and negative regulators for their gene targets. Although the tumor-suppressive functions of several miRNAs have been demonstrated, the dysregulation of miRNAs and their molecular mechanisms in endometrial cancer still remain unclear. To identify tumor-suppressive miRNAs (TS-miRs) silenced by DNA hypermethylation during endometrial carcinogenesis, we performed functional screening for TS-miRs in endometrial cancer cell lines. We further analyzed DNA hypermethylation of CpG-islands around mature miRNAs, resulting in identification of some miRNAs as the candidate TS-miRs silencing by CpG island hypermethylation. Our data suggest that these TS-miRs might play an important role in endometrial carcinogenesis.

MicroRNA (miRNA) 是小型非编码 RNA 及其基因靶标的负调节因子。尽管多种miRNA的抑癌功能已被证实，但子宫内膜癌中miRNA的失调及其分子机制仍不清楚。为了鉴定子宫内膜癌发生过程中因 DNA 高甲基化而沉默的肿瘤抑制 miRNA (TS-miR)，我们对子宫内膜癌细胞系中的 TS-miR 进行了功能筛选。我们进一步分析了成熟 miRNA 周围 CpG 岛的 DNA 高甲基化，从而确定了一些 miRNA 作为 CpG 岛高甲基化沉默的候选 TS-miR。我们的数据表明这些 TS-miR 可能在子宫内膜癌发生中发挥重要作用。

项目成果

Banno K, Kobayashi Y, Yanokura M, Kishimi A, Ogawa S, Kisu I, Nomura H, Hirasawa A, Susumu N, Aoki D.

Banno K、小林 Y、Yanokura M、Kishimi A、小川 S、Kisu I、野村 H、平泽 A、Susumu N、青木 D。

• 发表时间: 2009
• 期刊: J Fam Tumor 9
• 作者: Muraki Y;Banno K;Yanokura M;Kobayashi Y;Kawaguchi M;Nomura H;Hirasawa A;Susumu N;Aoki D.;Endomatrial cancer as HNPCC associated tumor
• 通讯作者: Endomatrial cancer as HNPCC associated tumor

Endomatrial cancer as HNPCC associated tumor.

子宫内膜癌为 HNPCC 相关肿瘤。

• 发表时间: 2009
• 期刊: J Fam Tumor 9
• 作者: Banno K;Yanokura M;Kobayashi Y;Kawaguchi M;Nomura H;Hirasawa A;Susumu N;Aoki D.;Kawaguchi M;Muraki Y;Kawaguchi M;Banno K
• 通讯作者: Banno K

Nishimura S, Tsuda H, Miyagi Y, Hirasawa A, Suzuki A, Kataoka F, Nomura H, Chiyoda T, Banno K, Fujii T, Susumu N, Aoki D.

西村 S、津田 H、宫城 Y、平泽 A、铃木 A、片冈 F、野村 H、千代田 T、班诺 K、藤井 T、Susumu N、青木 D。

• 发表时间: 2008
• 期刊: Br J Cancer 99
• 作者: Sugano K;Nakamura S;Ando J;Takayama S;Kamata H;Sekiguchi I;Ubukata M;Kodama T;Arai M;Kasumi F;Hirai Y;Ikeda T;Jinno H;Kitajima M;Aoki D;Hirasawa A;Takeda Y;Yazaki K;Fukutomi T;Kinoshita T;Tsunematsu R;Yoshida T;Izumi M;Umezawa S;Ya;Can ABCF2 protein expression predict the prognosis of uterine cancer?
• 通讯作者: Can ABCF2 protein expression predict the prognosis of uterine cancer?

Pharmacogenomic effects of UDP-glucuronosyltransferase 1A1 on irinotecan-induced drug reaction and serum bilirubin levels

UDP-葡萄糖醛酸基转移酶1A1对伊立替康诱导的药物反应和血清胆红素水平的药物基因组学影响

• 发表时间: 2009
• 作者: Hirasawa A;Akahane T;Tsuruta T;Nomura H;Banno K;Tsuda H;Saito K;Zama T;Tanigawara Y;Susumu N;Aoki D.
• 通讯作者: Aoki D.

遺伝性乳がん・卵巣がんの遺伝子検査実施後の状況と遺伝相談外来の役割

遗传性乳腺癌和卵巢癌基因检测后的状况以及遗传咨询门诊的作用

• 发表时间: 2008
• 作者: Hirasawa A;Tsuruta T;Nomura H;Kataoka F;Tominaga E;Banno K;Suzuki A;Tsuda H;Naganishi H;Saito K;Zama T;Tanigawara Y;Susumu N;Aoki D;平沢晃;堀場裕子;牧田和也;堀場裕子;片岡史夫;野村弘行;市川義一;平沢晃;鶴田智彦;冨永英一郎;武田祐子
• 通讯作者: 武田祐子