The mechanism of water absorption in the endolymphatic sac

内淋巴囊吸水的机制

• 批准号: 20791179
• 负责人: NAKAYA Kazuhiro
• 金额: $ 2.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791179/
• 关键词: RNAi; 内リンパ水腫

AQP2 mRNA was knocked down by placing siRNA specific to rat AQP2 at the round window membrane. The samples were harvested 48 hours after the intervention. The immunohistochemical study under the fluorescent microscope revealed that the expression of the AQP2 in the endolymphatic sac was reduced in the group treated with AQP2 siRNA compared to the group treated with negative control siRNA. Neither endolymphatic hydrops nor endolymphatic collapse occurred in both groups.

通过将大鼠 AQP2 特异性的 siRNA 放置在圆窗膜上，AQP2 mRNA 被敲低。干预后 48 小时采集样本。荧光显微镜下的免疫组织化学研究显示，与阴性对照siRNA处理组相比，AQP2 siRNA处理组内淋巴囊中AQP2的表达降低。两组均未发生内淋巴水肿或内淋巴塌陷。