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Severe Combined Immunodeficient(SCID) Mice As Animal Models for Human Echinococcosis

Severe Combined Immunodeficient(SCID) Mice As Animal Models for Human Echinococcosis
严重联合免疫缺陷(SCID)小鼠作为人类包虫病的动物模型
批准号:
15500295
负责人:
NAKAYA Kazuhiro
金额:
$2.43万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2004

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中文摘要
翻译
本研究首先比较了两株严重联合免疫缺陷小鼠C.B-17/Icr-scid和NOD/Shi-scid对泡球蚴的敏感性。于感染后9周和12周,对两株菌腹腔下发育的后囊蚴进行称重和比较。NOD/Shi-scid在两周内均比C.B-17/Icr-scid表现出更高程度的增重。结果表明,scid基因的表达与巨噬细胞功能和补体活性的下降共同影响NOD/Shi-scid小鼠后绦虫的发育和生长。感染后1、2、3周,NOD/Shi-scid小鼠和对照NOD/Shi-+/+小鼠的后绦虫重量和形态均无明显差异。NOD/Shi-+/+小鼠在这些时间段内未发现rEm-18抗体。认为这是一个尚未产生抗体的潜伏期。感染后6周,NOD/Shi-scid小鼠迅速发生多囊化和原头节形成。NOD/Shi-scid小鼠对寄生虫的生长发育具有明显的耐受性。作者认为NOD/Shi-scid小鼠可作为人体包虫病的动物模型,并对NOD/Shi-scid小鼠与NOD/Shi-+/+小鼠之间的多囊蚴形成和原头节形成进行了定量研究。感染后6周,NOD/Shi-scid小鼠多囊化指数和原头节形成指数突然增加。而NOD/Shi-+/+小鼠,尽管寄生虫和炎症性墨角藻扩散,但多囊泡指数降低,而囊泡数量不变。NOD/Shi-scid小鼠的原头节指数也比NOD/Shi-+/+小鼠增加。因此,建立寄生虫生长发育指数的方法,可作为评价宿主敏感性、药物疗效、判断感染点的参考。
英文摘要
At first, two strains of sever combined immune-deficiency mice, C.B-17/Icr-scid and NOD/Shi-scid were compared with the sensitivity to Echinococcus multilocularis metacestode. At post infection 9 and 12weeks, the developed metacestode of two strains infra peritoneal cavities were weighed and compared. NOD/Shi-scid showed more high degree weight than C.B-17/Icr-scid at both points of weeks. It was suggested that scid-gene, declines of macrophage function and compliment activity were influenced together.So, development and growth of metacestode in NOD/Shi-scid were observed in detail. At post infection 1,2,3 weeks, between NOD/Shi-scid and control NOD/Shi-+/+ mice, there were no defferences both weight and morphologies of metacestodes. NOD/Shi-+/+ mice were not revealed for rEm-18 antibody in these periods. It was considered that so-called prepatent period which antibody was not yet producted in host immune system. After post infection 6 weeks, in NOD/Shi-scid mice, multivesiculation and formation of protoscolex were rapidly proceeded. NOD/Shi-scid mice showed striking torelance for the parasite development and growth. It was suggested that NOD/Shi-scid mouse would be a model for human echinococcosis.And moreover, it was attempted of quantification of multivesiculation and protoscolex formation of the parasite between NOD/Shi-scid and NOD/Shi-+/+ mice. After post infection 6 weeks, in NOD/Shi-scid mice, index of multivesiculation and protoscolex formation suddenly increased. While in NOD/Shi-+/+ mice, in spite of spread of parasite and inflamated fucus, index of multivesicelation was decreased for not changed number of vesicular. Index of protoscolex also increased more in NOD/Shi-scid mice than in NOD/Shi-+/+ mice. It was expected that index methods of these parasite development or growth was usefullness in evaluation of host sensitivity and drug efficacy, estimate of infectious point.
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DOI: 10.1128/jcm.42.3.1082-1088.2004
发表时间: 2004-03-01
期刊: JOURNAL OF CLINICAL MICROBIOLOGY
影响因子: 9.4
作者: [Mamuti, W, Yamasaki, H, Ito, A]
通讯作者: Ito, A
伊藤亮, 石川裕司, 北田正博, 中谷和宏, 笹嶋唯博(2003): "肺エキノコックス症"呼吸. 22・1. 56-60 (2003)
Ryo Ito、Yuji Ishikawa、Masahiro Kitada、Kazuhiro Nakatani、Yuhiro Sasashima (2003):“肺包虫病”22・1 (2003)。
DOI: --
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作者: []
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发表时间: 2003-12
期刊: Comparative medicine
影响因子: 0.8
作者: [T. Asanuma;Yukari Matsumoto;M. Takiguchi;O. Inanami;M. Nakao;K. Nakaya;A. Ito;A. Hashimoto;M. Kuwabara]
通讯作者: T. Asanuma;Yukari Matsumoto;M. Takiguchi;O. Inanami;M. Nakao;K. Nakaya;A. Ito;A. Hashimoto;M. Kuwabara
Ito A, Sako Y, Yamasaki H, Mamuti W, Nakaya K, Nakao M, Ishikawa Y.: "Development of Em18-immunoblot and Em18-ELISA for specific diagnosis of alveolar echinococcosis"Acta Trop.. 85・2. 173-182 (2003)
Ito A、Sako Y、Yamasaki H、Mamuti W、Nakaya K、Nakao M、Ishikawa Y.:“开发用于特异性诊断肺泡包虫病的 Em18 免疫印迹和 Em18-ELISA”Acta Trop.. 85・2。 (2003)
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