Severe Combined Immunodeficient(SCID) Mice As Animal Models for Human Echinococcosis

首先比较两株严重联合免疫缺陷小鼠C.B-17/Icr-scid和NOD/Shi-scid对多房棘球蚴的敏感性。在感染后9周和12周，对两株腹膜下腔发育的跖骨肿进行称重比较。在两个星期的时间点，NOD/Shi-scid表现出比C.B-17/Icr-scid更高的度重。提示scid基因、巨噬细胞功能下降和补体活性共同受到影响。因此，我们详细观察了NOD/Shi-scid中metacestode的发育和生长情况。在感染后1、2、3周，NOD/Shi-scid与对照组NOD/Shi-+/+小鼠之间的体重和跖骨形态均无差异。NOD/Shi-+/+小鼠在这些时期未发现rEm-18抗体。被认为是在宿主免疫系统中尚未产生抗体的所谓pre - patent期。感染6周后，NOD/Shi-scid小鼠多囊化和原头节形成迅速进行。NOD/Shi-scid小鼠对寄生虫的发育和生长表现出惊人的耐受性。提示NOD/ shiscid小鼠可作为人类棘球蚴病的实验模型。此外，还尝试定量测定NOD/Shi-scid和NOD/Shi-+/+小鼠间寄生虫的多囊化和原头节形成。感染后6周，NOD/Shi-scid小鼠多泡指数和原头节形成指数突然升高。而NOD/Shi-+/+小鼠，尽管有寄生虫和炎症菌体的扩散，但囊泡数不变，多囊化指数下降。NOD/Shi-scid组原头节指数比NOD/Shi-+/+组增加更多。期望这些寄生虫发育或生长的指数方法可用于评价宿主敏感性和药物疗效，估计感染点。

At first, two strains of sever combined immune-deficiency mice, C.B-17/Icr-scid and NOD/Shi-scid were compared with the sensitivity to Echinococcus multilocularis metacestode. At post infection 9 and 12weeks, the developed metacestode of two strains infra peritoneal cavities were weighed and compared. NOD/Shi-scid showed more high degree weight than C.B-17/Icr-scid at both points of weeks. It was suggested that scid-gene, declines of macrophage function and compliment activity were influenced together.So, development and growth of metacestode in NOD/Shi-scid were observed in detail. At post infection 1,2,3 weeks, between NOD/Shi-scid and control NOD/Shi-+/+ mice, there were no defferences both weight and morphologies of metacestodes. NOD/Shi-+/+ mice were not revealed for rEm-18 antibody in these periods. It was considered that so-called prepatent period which antibody was not yet producted in host immune system. After post infection 6 weeks, in NOD/Shi-scid mice, multivesiculation and formation of protoscolex were rapidly proceeded. NOD/Shi-scid mice showed striking torelance for the parasite development and growth. It was suggested that NOD/Shi-scid mouse would be a model for human echinococcosis.And moreover, it was attempted of quantification of multivesiculation and protoscolex formation of the parasite between NOD/Shi-scid and NOD/Shi-+/+ mice. After post infection 6 weeks, in NOD/Shi-scid mice, index of multivesiculation and protoscolex formation suddenly increased. While in NOD/Shi-+/+ mice, in spite of spread of parasite and inflamated fucus, index of multivesicelation was decreased for not changed number of vesicular. Index of protoscolex also increased more in NOD/Shi-scid mice than in NOD/Shi-+/+ mice. It was expected that index methods of these parasite development or growth was usefullness in evaluation of host sensitivity and drug efficacy, estimate of infectious point.