Cardiomyogenesis and the Mechanism of Immunologic Tolerance of Human Amniotic Mesenchymal Stem Cell

人羊膜间充质干细胞的心肌发生及免疫耐受机制

• 批准号: 20791175
• 负责人: NISHIYAMA Hiroko
• 金额: $ 2.75万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791175/
• 关键词: 生科学; 生殖医療; 羊膜幹細胞; バイオマテリアル

Mesenchymal stem cells (MSCs) have recently been used for clinical application, and their safety and feasibility in cardiac stem cell-based therapy have been demonstrated, specially human bone marrow-derived MSCs (BMMSCs). Thus, MSCs are a more important cellular source for stem cell-based therapy from a practical point of view. However there are several problems. The efficacy of BMMSCs was still limited, however, because of low efficiency for cardiomyogenic transdifferentiation. The establishment of stem cell bank system and the use of immunosuppressant agents were also essential. While human amniotic membrane-derived mesenchymal stem cells (hAMCs) is known to differentiate into many tissues in vitro. Moreover human amniotic membrane is thought to play an important role for immunologic tolerance during pregnancy with expression of HLA-G, the non-classical human lerukocyte antigen (HLA) class I b. In the present study, we aimed to show hAMCs has a potency of cardiomyocyte differentiation, transplanted hAMCs-induced immunological tolerance and survival of transdifferentiated cardiomyocyte from HAMC in vivo.

间充质干细胞（MSCs）最近被用于临床应用，其在心脏干细胞治疗中的安全性和可行性已经得到证实，特别是人骨髓来源的MSCs （BMMSCs）。因此，从实用的角度来看，间充质干细胞是干细胞治疗更重要的细胞来源。然而，有几个问题。然而，由于成心肌转分化效率较低，BMMSCs的疗效仍然有限。干细胞库系统的建立和免疫抑制剂的使用也是必不可少的。而人羊膜源间充质干细胞（hamc）在体外可以分化为多种组织。此外，人羊膜被认为在妊娠期间的免疫耐受中发挥重要作用，表达HLA- g，非经典人白细胞抗原（HLA） I类b。在本研究中，我们旨在证明HAMC具有心肌细胞分化的能力，移植的HAMC诱导的免疫耐受以及从HAMC转分化的心肌细胞在体内的存活。

项目成果

腹腔鏡下にて治療可能であった卵管間質部妊娠の3例

腹腔镜治疗输卵管间质妊娠三例

• 发表时间: 2010
• 作者: 辻紘子;伊藤嘉佑子;古谷正敬;内田浩;浜谷敏生;浅田弘法;丸山哲夫;久慈直昭;末岡浩;吉村泰典
• 通讯作者: 吉村泰典

'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells

• DOI: 10.1016/j.yexcr.2007.04.028
• 发表时间: 2007-07-15
• 期刊: EXPERIMENTAL CELL RESEARCH
• 影响因子: 3.7
• 作者: Okamoto, Kazuma;Miyoshi, Shunichiro;Umezawa, Akihiro
• 通讯作者: Umezawa, Akihiro

子宮内膜症の発症に対して、EP300遺伝子多型が及ぼす影響

EP300基因多态性对子宫内膜异位症发生发展的影响

• 发表时间: 2009
• 期刊: 日本エンドメトリオーシス学会会誌 30
• 作者: 辻紘子;浅田弘法;浅井哲;古谷正敬;内田 浩;浜谷敏生;丸山哲夫;小崎健次郎;柳橋達彦;羽田智則;安藤正明;田島敏秀;木挽貢慈;吉村泰典
• 通讯作者: 吉村泰典

術前の腫瘍マーカーCA546の値は卵巣漿液性腺癌の予後を推定する因子となりうる:辻紘子(慶應義塾大学産婦人科)

术前肿瘤标志物CA546值可作为估计卵巢浆液性腺癌预后的一个因素：Hiroko Tsuji（庆应义塾大学妇产科）

• 发表时间: 2004
• 期刊: 日本産科婦人科学会関東連合地方部会会報(0285-8096) 41巻3号
• 作者: 青木大輔;野村弘行;鈴木直;富田明代;平尾健;江澤佐知子;玉田裕;鈴木淳;進伸幸;吉村泰典;野澤志朗
• 通讯作者: 野澤志朗

Xenografted Human Amniotic Membrane-Derived Mesenchymal Stem Cells were Immunologically Tolerated and Transdifferentiated Into Cardiomyocytes

异种移植的人羊膜来源的间充质干细胞具有免疫耐受性并转分化为心肌细胞

• 发表时间: 2010
• 期刊: Circulation Research
• 影响因子: 20.1
• 作者: Hiroko Tsuji;Shunichiro Miyoshi;Yukinori Ikegami;Naoko Hida;Hironori Asada;Ikuko Togashi;Junshi Suzuki;Masaki Satake;Hikaru Nakamizo;Mamoru Tanaka;Taisuke Mori;Kaoru Segawa;Nobuhiro Nishiyama;Junko Inoue;Hatsune Makino;Kenji Miyado;Satoshi
• 通讯作者: Satoshi