Study on intractable otitis media caused by Nontypeable Haemophilus influenza (NTHi) biofilm formation

非分型流感嗜血杆菌(NTHi)在体内可形成生物膜。生物膜被定义为一组细菌，作为一个群落生长在自己产生的聚合物基质中。多项研究表明，形成生物膜的能力有助于NTHi引起的急性中耳炎的持续。用96孔针复制器和激光共聚焦扫描显微镜评价NTHi的生物被膜形成和粘附性。生物膜中的细菌对抗生素高度耐药。

Nontypeable Haemophilus influenza (NTHi) has been shown to form biofilm in vivo. The biofilm is defined as a group of bacteria growing as a community in a self- produced polymeric matrix. Several studies show that the ability to form biolilms contributes the persistence of acute otitis media caused by NTHi. The biofilm formations and adherence of NTHi were evaluated by a 96-well pin-replicator assay with a confocal laser scanning microscopy. Bacteria in biofilms were highly resistant to antibiotics.