The elucidation of the onset organization of the trigeminal neuralgia using a trigeminal nerve damage animal.

使用三叉神经损伤动物阐明三叉神经痛的发病组织。

• 批准号: 20791562
• 负责人: ABE Tetsuya
• 金额: $ 2.75万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791562/
• 关键词: 三叉神経; 機械刺激受容ニューロン; サイトカイン; サブスタンスP; GABAAc-Fos; 三叉神経 主感覚核; GABAA; c-Fos; ニューロキニン1; SPサポリン; ニユーロン; ビククリン; GABA受容体; 侵害受容ニューロン

The orofacial region has skin and mucous region and the formalin injection into the two different region induces different nocifensive behavior. The subcutaneous injection of diluted formalin into the rat upper lip induced more nocifensive behaviour than into the tongue. The behavior was inhibited more by the preadministration of an antagonist of GABA_A receptor, bicuculline, when formalin was injected into the lip than into the tongue. The resultant differences of nocifensive behaviour and pharmacology could be caused by the differences in the quantitative and qualitative distribution of C-fiber population. The tongue has fewer primary afferent neurons (trigeminal nerve) in the range of C-fiber than the lip skin.Considerable number of neurons-bearing receptors of substance P (SP), neurokinin-1 (NK-1) are distributed in lamina I and less in lamina III of trigeminal subnucleus caudalis (Vc). We have eliminated these NK-1-bearing neurons by preadministration of SP conjugated with a ribosome-inactivating toxin, saporin (SP-Sap) into the rat cisterna magna. In these SP-Sap-pretreated rats, the formalin-induced nocifensive behaviour was reduced and the preadministration of bicuculline enhanced the nocifensive behaviour, when bicuculline reduced it in the naive rats. These results show that NK-1-bearing neurons are involved in pro-nociceptive transducer through ascending and descending nociceptive facilitating loop, where GABA acts as prpnociceptive.

口面部有皮肤和粘膜两个区域，福尔马林注射到这两个不同的区域引起不同的伤害反应行为。将稀释的福尔马林皮下注射到大鼠上唇中比注射到舌头中诱导更多的伤害性行为。预先给予GABA_A受体拮抗剂荷包牡丹碱对福尔马林口唇注射的抑制作用大于舌注射。由此产生的伤害反应行为和药理学的差异可能是由C-纤维群体的数量和质量分布的差异引起的。舌C纤维区三叉神经的初级传入神经元比唇皮少，三叉神经尾侧亚核（Vc）的第Ⅰ层和第Ⅲ层有较多的P物质（SP）、神经激肽-1（NK-1）受体。我们已经消除了这些NK-1轴承神经元预先管理SP共轭核糖体失活毒素，皂草素（SP-Sap）到大鼠枕大池。在这些SP-Sap预处理的大鼠中，福尔马林诱导的疼痛反应行为减少，预先给药荷包牡丹碱增强了疼痛反应行为，而荷包牡丹碱降低了幼稚大鼠的疼痛反应行为。这些结果表明，NK-1阳性神经元通过上行和下行伤害性促进环参与伤害性感受的传导，其中GABA起伤害性感受的作用。

项目成果

三叉神経慢性切断ラットの三叉神経節電気刺激が誘導する三叉神経感覚核のc-Fos発現

电刺激三叉神经节诱导慢性三叉神经横断大鼠三叉神经感觉核中c-Fos的表达

• 发表时间: 2009
• 作者: 阿部徹也;本田公亮;山村倫世;藤原正識;浦出雅裕;奥井森
• 通讯作者: 奥井森

Effects of systemic bicuculline or morphine on formalin-evoked pain-related behaviour and c-Fos expression in trigeminal nuclei after formalin injection into the lip or tongue in rats

• DOI: 10.1007/s00221-009-1842-1
• 发表时间: 2009-05
• 期刊: Experimental Brain Research
• 影响因子: 2
• 作者: S. Sugiyo;D. Uehashi;F. Satoh;Tetsuya Abe;Norifumi Yonehara;Masayuki Kobayashi;M. Takemura

ラットにおける下歯槽神経、眼窩下神経切断後に三叉神経節への電気刺激が誘導する三叉神経感覚核の異常なc-Fos発現

大鼠下牙槽神经和眶下神经横断后电刺激三叉神经节致三叉神经感觉核c-Fos异常表达

• 发表时间: 2009
• 作者: 阿部徹也;本田公亮;山村倫世;奥井森;浦出雅裕
• 通讯作者: 浦出雅裕

Effect of systemic bicuculine or morphine on formarin-evoked pain-related behaviour and c-Fos expression in trigeminal nuclei after formalin injection into the lip or tongue in rats.

福尔马林注射到大鼠唇或舌后，全身荷包牡丹碱或吗啡对福尔马林诱发的疼痛相关行为和三叉神经核 c-Fos 表达的影响。

• 发表时间: 2009
• 期刊: Exp Brain Res 196
• 作者: S. Sugiyo;D. Uehashi;F. Satoh;T. Abe;N. Yonehara;M. Kobayashi;Motohide Takemura.
• 通讯作者: Motohide Takemura.

Neurokinin-1-receptor bearing neurons in the trigeminal caudal nucleus are crucial for intensity-dependent bidirectional sensory processing through GABAA receptors

三叉神经尾核中带有 Neurokinin-1 受体的神经元对于通过 GABAA 受体进行强度依赖性双向感觉处理至关重要

• 发表时间: 2008
• 作者: 阿部徹也;本田公亮;山村倫世;藤原正識;浦出雅裕;奥井森;阿部徹也
• 通讯作者: 阿部徹也