Mass Spectrometer(high-resolution LC-ESI-IMS-MSMS Spectrometer)

质谱是一种交叉技术，在化学、生物化学和分子生物学研究和教学中起着关键作用。波茨坦大学化学研究所和生物化学与生物学研究所的代表联合起来，将迫切需要的更换和新购置的单个质谱仪与建立跨研究所MS分析中心相结合。我们的目标是加强协同效应，分享和扩大波茨坦大学的分析专业知识。此外，我们打算协调各种分析技术的使用，以提高总体能力，防止出现维修瓶颈。本课题主要应用于化学研究所的液相色谱-电喷雾质谱联用仪（LC-ESI-IMS-MSMS）对溶解样品进行分析，一方面取代了易维修的ESI-MSMS质谱仪，另一方面为离子迁移谱提供了一种新的正交分析方法。该仪器将用于对小分子、极性分子以及肽、蛋白质及其复合物进行高分辨率ESI测量。作为一揽子提案的一部分，还要求增加下列设备：1）GC-EI-MSMS仪器，用于小的、容易蒸发的分析物（替换提案/化学研究所），2）MALDI-MSMS仪器，其中样品以结晶基质形式施加到该仪器中（替换提案/生物化学和生物学研究所），和3）用于溶解样品的超高分辨率LC-ESI-Orbitrap-MSMS仪器（新的收购建议/生物化学和生物研究所）。这两个研究所的许多团体将受益于仪器的更高性能和多功能性（包括国际监测系统和超高分辨率）以及显著增加的样品容量，将能够推进具有国际竞争力的研究项目。

Mass spectrometry is a cross-sectional technology that plays a key role in chemical, biochemical and molecular biological research and teaching. Representatives of the Institute of Chemistry and the Institute of Biochemistry and Biology of the University of Potsdam have joined forces and combined the urgently needed replacements and a new acquisition of individual mass spectrometry instruments with the establishment of a cross-institute MS analytics center. Our aim is to strengthen synergy effects and to share and expand analytical expertise at the University of Potsdam. Further, we intend to coordinate the use of various analytical techniques, to enhance the overall capacity and to prevent repair bottlenecks. With main use in the Institute of Chemistry, here, we apply for an LC-ESI-IMS-MSMS instrument for the analysis of dissolved samples, on the one hand to replace the old ESI-MSMS spectrometer, which is prone to repair, and on the other hand to provide ion mobility spectrometry as a new, orthogonal method. The instrument will be used to perform high-resolution ESI measurements on small, polar molecules but also on peptides, proteins and their complexes. As part of the package proposal, the following additional equipment is also requested:1) a GC-EI-MSMS instrument for small, easily vaporizable analytes (replacement proposal / Institute of Chemistry), 2) a MALDI-MSMS instrument into which samples are applied in crystalline matrix (replacement proposal / Institute of Biochemistry and Biology), and 3) an ultra-high resolution LC-ESI-Orbitrap-MSMS instrument for dissolved samples (new acquisition proposal / Institute of Biochemistry and Biology).Numerous groups from both institutes will benefit from higher performance and versatility of the instruments (including IMS and ultra-high resolution) as well as the significantly increased sample capacity and will be able to advance internationally competitive research projects.