Structural analysis of biomolecules via high-energy collision Induced dissociation using a Miniaturized TOFMS

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A multi-turn tandem time-of-flight (TOF) mass spectrometer (MULTUM-TOF/TOF) was used in this study. The instrument has two features. One is high precursor ion selectivity of the first TOF, which has much longer flight distance. Another is the elimination of fragment ions via post-source decay process. Components of post-source decay change its kinetic energy, therefore they cannot path through electric sectors. We performed the structural analysis of biomolecules using high-energy collision induced dissociation (HE-CID). I confirmed that I could obtain a lot of useful information to determine precise structure of biomolecules with small quantity of analyte. The HE-CID using MULTUM-TOF/TOF is a powerful tool to determine structure of novel bioactive substances in the future.