Cellular analysis of the plant organ and organ boundary formation

植物器官和器官边界形成的细胞分析

• 批准号: 20870029
• 负责人: TAKEDA Seiji
• 金额: $ 2.11万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20870029/
• 关键词: シロイヌナズナ; 胚発生; 器官形成; 器官境界部; CUP-SHAPED COTYLEDON; ALOG遺伝子ファミリー; 茎頂分裂組織; イメージング; 蛍光タンパク質; 細胞動態

Plants differentiate boundary cells between organs to separate their cellular fates. To understand the molecular mechanism of organ boundary formation, I performed (1) visualization of boundary cells by using the fluorescent protein and boundary-specific genes, and (2) functional analysis of a boundary determinant CUC1 and its transcriptional targets. Two members of the ALOG gene family, LSH4 and LSH3, are direct transcriptional targets of CUC1, and both of them may be involved in meristem formation and organ separation. The ectopic expression of CUC1 resulted in the transformation of ovules to stigmatic tissue, suggesting that it controls organ identity.

植物区分器官之间的边界细胞以区分它们的细胞命运。为了了解器官边界形成的分子机制，我进行了 (1) 使用荧光蛋白和边界特异性基因对边界细胞进行可视化，以及 (2) 对边界决定子 CUC1 及其转录靶标进行功能分析。 ALOG基因家族的两个成员LSH4和LSH3是CUC1的直接转录靶标，它们都可能参与分生组织形成和器官分离。 CUC1 的异位表达导致胚珠转变为柱头组织，表明它控制器官特性。

项目成果

Functional Analysis of LIGHT-DEPENDENT SHORT HYPOCOTYLES of Which Transcription Is Controlled by Organ Boundary Determinants CUP-SHAPED COTYLEDON Genes

转录受器官边界决定子杯形子叶基因控制的光依赖性短下胚轴的功能分析

• 发表时间: 2008
• 作者: Seiji Takeda;Keiko Hanano;Yuka Tsubakimoto;Ayano Kariya;Satoko Shimizu;Masao Tasaka;Mitsuhiro Aida
• 通讯作者: Mitsuhiro Aida

Functional analysis of direct target genes of CUC1 which controls organ boundary formation in Arabidopsis thaliana

拟南芥CUC1控制器官边界形成直接靶基因的功能分析

• 发表时间: 2009
• 作者: Seiji Takeda;Keiko Hanano;Yuka Tsubakimoto;Ayano Kariya;Satoko Shimizu;Masao Tasaka;Mitsuhiro Aida
• 通讯作者: Mitsuhiro Aida

Functional analysis of idrect target genes of CUC1 which controls organ boundary formation in Arabidopsis thaliana

拟南芥CUC1控制器官边界形成的iddirect靶基因的功能分析

• 发表时间: 2009
• 作者: Seiji Takeda;Keiko Hanano;Yuka Tsubakimoto;Ayano Kariya;Satoko Shimizu;Masao Tasaka;Mitsuhiro Aida
• 通讯作者: Mitsuhiro Aida

Functional analysis of LSH genes that are direct targets of CUC1 transcription factor controlling organ boundary formation

作为控制器官边界形成的 CUC1 转录因子直接靶标的 LSH 基因的功能分析

• 发表时间: 2009
• 作者: Seiji Takeda;Keiko Hanano;Yuka Tsubakimoto;Ayano Kariya;Satoko Shimizu;Masao tasaka;Mitsuhiro Aida
• 通讯作者: Mitsuhiro Aida

器官境界部を作るCUC1遺伝子の下流遺伝子LSH4の機能解析

LSH4（CUC1 基因下游基因，创建器官边界）的功能分析

• 发表时间: 2009
• 作者: 武田征士;花野恵子;椿本有雅;苅谷綾乃;清水聡子;田坂昌生;相田光宏
• 通讯作者: 相田光宏