Cellular analysis of the plant organ and organ boundary formation

植物分化器官之间的边界细胞，以分开它们的细胞命运。为了理解器官边界形成的分子机制，我进行了（1）使用荧光蛋白和边界特异性基因的边界细胞可视化，以及（2）边界决定子CUC 1及其转录靶点的功能分析。ALOG基因家族的两个成员LSH 4和LSH 3是CUC1的直接转录靶点，它们都可能参与分生组织的形成和器官的分离。CUC1的异位表达导致胚珠向柱头组织的转化，这表明它控制器官的身份。

Plants differentiate boundary cells between organs to separate their cellular fates. To understand the molecular mechanism of organ boundary formation, I performed (1) visualization of boundary cells by using the fluorescent protein and boundary-specific genes, and (2) functional analysis of a boundary determinant CUC1 and its transcriptional targets. Two members of the ALOG gene family, LSH4 and LSH3, are direct transcriptional targets of CUC1, and both of them may be involved in meristem formation and organ separation. The ectopic expression of CUC1 resulted in the transformation of ovules to stigmatic tissue, suggesting that it controls organ identity.