Development of glycopeptide array aiming for diagnosis of infectious diseases

开发用于感染性疾病诊断的糖肽芯片

• 批准号: 20890007
• 负责人: KATO Kentaro
• 金额: $ 2.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20890007/
• 关键词: 糖ペプチドアレイ; レクチン; 感染症

MUC2 mucin (glyco)peptides with fifty-six patterns of N-acetylgalactosamine (GalNAc) residue attachment were synthesized and immobilized on the plate. Binding affinities of VVA-B4, a plant lectin, toward GalNAc attached MUC2 peptides were measured using ELISA. The results suggested that the affinity was defined by the sites of GalNAc attachment on the peptides. The plate with GalNAc attached MUC2 peptides was successfully developed and this made possible for measuring the affinities of parasite lectins toward GalNAc attached MUC2 peptides.

合成了具有56种n -乙酰半乳糖胺（GalNAc）残基附着模式的MUC2粘蛋白（糖）肽，并将其固定在平板上。采用ELISA法测定了植物凝集素VVA-B4与GalNAc粘附的MUC2肽的结合亲和力。结果表明，亲和性是由GalNAc附着在肽上的位置决定的。成功构建了附着GalNAc的MUC2肽板，使测定寄生虫凝集素对附着GalNAc的MUC2肽的亲和力成为可能。

项目成果

生体におけるムチン型糖鎖の役割

粘蛋白型糖链在生物体中的作用

• 发表时间: 2010
• 期刊: 第3回原虫感染免疫研究会
• 作者: 山岨達也;樫尾明憲;坂本幸士;岩本均;近藤嘉高;石神昭人;加藤健太郎
• 通讯作者: 加藤健太郎