Development of glycopeptide array aiming for diagnosis of infectious diseases

合成了具有56种n -乙酰半乳糖胺（GalNAc）残基附着模式的MUC2粘蛋白（糖）肽，并将其固定在平板上。采用ELISA法测定了植物凝集素VVA-B4与GalNAc粘附的MUC2肽的结合亲和力。结果表明，亲和性是由GalNAc附着在肽上的位置决定的。成功构建了附着GalNAc的MUC2肽板，使测定寄生虫凝集素对附着GalNAc的MUC2肽的亲和力成为可能。

MUC2 mucin (glyco)peptides with fifty-six patterns of N-acetylgalactosamine (GalNAc) residue attachment were synthesized and immobilized on the plate. Binding affinities of VVA-B4, a plant lectin, toward GalNAc attached MUC2 peptides were measured using ELISA. The results suggested that the affinity was defined by the sites of GalNAc attachment on the peptides. The plate with GalNAc attached MUC2 peptides was successfully developed and this made possible for measuring the affinities of parasite lectins toward GalNAc attached MUC2 peptides.