Elucidation of malaria erythrocyte invasion system using stem cell techonology

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We confirmed the invasion of Plasmodium falciparum using erythrocyte like cells from human umbilical cord blood hematopoietic stem cell and mouse ES cells. The establishment of effective production system of mature erythrocyte from stem cell has been mainly proceeded. Before the treatment of ES cell and iPS cell, the technique that CD34 positive hematopoietic stem cell are isolated from umbilical cord blood and cultured have been mastered. Afterwards, we investigated the effective condition to lead enucleation with arrangement of class, concentration of cytokine, and culture period.Moreover, to produce the differentiated erythrocyte mutant with specific protein deficient, we tried to establish the trancfection system of differentiated erythrocyte using lentivirus vecter.