The Possibility of Cancer Diagnosis with Fluidity Sensitive Fluorescence Measurement

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BACKGROUND AND AIMS : The cellular membrane fluidity changes in response to physiological and pathological stimuli. We showed that the indomethacin treatment increased the fluidity of gastric cellular membrane using a specially designed microscope based system to measure the fluorescence polarization of gastric mucosa-derived RGM-1 cells stained with dodecanoylaminofluorescein (DAF). In the present study, the membrane fluidity of gastric cancer specimens and their surrounding normal mucosa were investigated whether carcinogenesis increased the cellular membrane fluidity. PATIENTS AND METHODS : Gastric cancer specimens from a total of 11 gastric cancer patients that were resectioned with Endoscopic Submucosal Dissection procedure were investigated. After washed with phosphate buffered saline, the specimens were topically treated with DAF and fluorescence polarization (FP) values on the surface cellular membrane were measured and calculated with an image intensified CCD-mounted fluorescence microscope and an image-processor. Diagnostic accuracy was evaluated and compared with pathological sections stained by H&E stain. RESULTS : The FP values of histologically-confirmed human astric cancer showed significantly smaller than those of surrounding normal gastric mucosa, indicating that cellular membrane fluidity was decreased in human cancer tissues. CONCLUSION : The present study revealed the cellular membrane fluidity increased during gastric carcinogenesis. The results exhibited the possibility of gastric cancer diagnosis with fluidity measurement.