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The Possibility of Cancer Diagnosis with Fluidity Sensitive Fluorescence Measurement

通过流体敏感荧光测量诊断癌症的可能性

基本信息

批准号：
20890029
负责人：
KANEKO Tsuyoshi
金额：
$ 1.97万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (Start-up)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

项目摘要

BACKGROUND AND AIMS : The cellular membrane fluidity changes in response to physiological and pathological stimuli. We showed that the indomethacin treatment increased the fluidity of gastric cellular membrane using a specially designed microscope based system to measure the fluorescence polarization of gastric mucosa-derived RGM-1 cells stained with dodecanoylaminofluorescein (DAF). In the present study, the membrane fluidity of gastric cancer specimens and their surrounding normal mucosa were investigated whether carcinogenesis increased the cellular membrane fluidity. PATIENTS AND METHODS : Gastric cancer specimens from a total of 11 gastric cancer patients that were resectioned with Endoscopic Submucosal Dissection procedure were investigated. After washed with phosphate buffered saline, the specimens were topically treated with DAF and fluorescence polarization (FP) values on the surface cellular membrane were measured and calculated with an image intensified CCD-mounted fluorescence microscope and an image-processor. Diagnostic accuracy was evaluated and compared with pathological sections stained by H&E stain. RESULTS : The FP values of histologically-confirmed human astric cancer showed significantly smaller than those of surrounding normal gastric mucosa, indicating that cellular membrane fluidity was decreased in human cancer tissues. CONCLUSION : The present study revealed the cellular membrane fluidity increased during gastric carcinogenesis. The results exhibited the possibility of gastric cancer diagnosis with fluidity measurement.

背景与目的：细胞膜流动性在生理和病理刺激下发生变化。我们发现，吲哚美辛治疗增加了胃细胞膜的流动性，使用一个专门设计的显微镜为基础的系统，以测量用十二烷酰氨基荧光素（dodecanoylaminofluorescein，DAF）染色的胃粘膜衍生的RGM-1细胞的荧光偏振。本研究探讨了胃癌组织及其周围正常粘膜细胞膜流动性是否随癌变而增加。患者和方法：研究了总共11例采用内窥镜粘膜下剥离术切除的胃癌患者的胃癌标本。用磷酸盐缓冲盐水洗涤后，用荧光素进行局部处理，用图像增强型CCD荧光显微镜和图像处理器测量并计算表面细胞膜上的荧光偏振（FP）值。并与H&E染色病理切片进行比较。研究结果：组织学证实的人胃癌的FP值显示出显著小于周围正常胃粘膜的FP值，表明人癌组织中细胞膜流动性降低。结论：胃癌发生过程中细胞膜流动性增加。结果表明，流动性测定用于胃癌诊断是可行的。