Analysis of inductive effect of mPOU on PRL cell differentiation for future application for pituitary tumor treatment

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Prolactinoma is a treatable pituitary tumor that responds to dopamine agonists. Induction for PRL cell differentiation may be related to PRL and D2 receptor gene expression and resulted in treatable tumors. In the present study, to clarify the mechanism of PRL gene expression, we searched brain cDNA library and found that mPOU bound to PRL gene and increased PRL reporter gene expression. mPOU also stimulated Pit-1 reporter gene expression. RNA interference for mPOU resulted in a decrease in PRL content and Pit-1 mRNA levels in GH3 cells. Chromatin immuno-precipitation assay showed that mPOU bound to PRL and Pit-1 gene. Taken together, these results suggested that mPOU was a stimulating factor for PRL gene expression directly and indirectly via enhancement of Pit-1 gene expression.