Studies on the neutrophil-mediated transport of Shiga toxins

肠出血性大肠杆菌（EHEC）是小儿出血性结肠炎的主要病原体，可引起溶血性尿毒综合征（HUS）和脑病。肠出血性大肠杆菌（EHEC）产生的滋贺毒素（Stx 1和Stx 2）可损伤高表达Gb3的靶细胞，但Stx从肠道向靶细胞的转运机制尚不清楚。在这项研究中，我们证明了Stxs结合到中性粒细胞中的LacCer，并绕过溶酶体和高尔基体在细胞核中积累。考虑到HUS患者肾小球中的中性粒细胞积聚，他们应该将Stx从肠道运输到靶细胞。

Enterohemorrhagic Escherichia coli (EHEC), a major pathogen of pediatric hemorrhagic colitis, cause hemolytic uremic syndrome (HUS) and encephalopathy. Shiga toxins (Stx1 and Stx2) produced by EHEC damage target cells which highly express Gb3, but the transport mechanism of Stxs from the intestinal tract to the target cells is unclear. In this study, we demonstrate that Stxs bind to LacCer in neutrophils and accumulate in the nucleus bypassing lysosomes and Golgi bodies. In view of neutrophil accumulation in glomeruli of patients with HUS, they should transport Stxs to target cells from the intestinal tract.