Asbestos fiber concentration in lung tissues and mutation analysis of malignant pleural mesothelioma

恶性胸膜间皮瘤肺组织石棉纤维浓度及突变分析

• 批准号: 20591665
• 负责人: YOKOI Kohei
• 金额: $ 3.08万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591665/
• 关键词: 悪性胸膜中皮腫; アスベスト; 遺伝子変異; 癌遺伝子; 癌抑制遺伝子; 肺内アスベスト濃度

We established twelve malignant pleural mesothelioma(MPM) cell lines from Japanese patients. We detected homozygous deletion of p16^<INK4A>/p14^<ARF> in all MPM cell lines. However, mutations of other tumor suppressor genes, including TP53, and protooncogenes, including KRAS, NRAS, BRAF, EGFR and HER2, were not found in these cell lines. Two women who had no history of asbestos exposure showed lower concentrations of crysotile and amphibole asbestos than the control subject. On the other hand, most patients with a history of asbestos exposure had higher concentrations of crysotile and amphibole asbestos than the control subject, but even in some patients with a history of asbestos exposure the low concentrations were observed. To elucidate the relation between the pulmonary asbestos fiber concentrations by fiber types and development of MPM, further investigations are necessary.

我们建立了12个恶性胸膜间皮瘤（MPM）细胞系从日本患者。我们在所有的MPM细胞系中检测到p16^ /p14^的纯合性缺失<INK4A><ARF>。然而，在这些细胞系中未发现其他肿瘤抑制基因（包括TP 53）和原癌基因（包括KRAS、NRAS、BRAF、EGFR和HER 2）的突变。两名没有石棉暴露史的妇女显示出比对照组低浓度的隐石棉和闪石石棉。另一方面，大多数有石棉暴露史的患者的隐石棉和闪石石棉浓度高于对照组，但即使在一些有石棉暴露史的患者中也观察到低浓度。为了阐明肺石棉纤维浓度的纤维类型和MPM的发展之间的关系，需要进一步的研究。

项目成果

Stepwise examination for differential diagnosis of primary lung cancer and breast cancer relapse presenting as a solitary pulmonary nodule in patients after mastectomy.

乳房切除术后患者的原发性肺癌和乳腺癌复发（表现为孤立性肺结节）的逐步检查鉴别诊断。

• 发表时间: 2008
• 期刊: J Surg Oncol 98
• 作者: Okasaka T;Usami N;Mitsudomi T;Yatabe Y;Matsuo K;Yokoi K.
• 通讯作者: Yokoi K.

Asbestos fiber concentration in lung tissues resected for malignant pleural mesothelioma

恶性胸膜间皮瘤切除肺组织中石棉纤维的浓度

• 发表时间: 2010
• 作者: Matsuguma H;Nakahara R;Ishikawa Y;Suzuki H;Inoue K;Katano S;Yokoi K.;Noriyasu Usami
• 通讯作者: Noriyasu Usami

Establishment and characterization of new malignant pleural mesothelioma cell lines from Japanese patients

来自日本患者的新恶性胸膜间皮瘤细胞系的建立和表征

• 发表时间: 2008
• 作者: Taniguchi T;Murakami H;Fujii M;Usami N;Yokoi K;Sekido Y
• 通讯作者: Sekido Y

Postoperative radiotherapy for patients with completely resected pathological stage IIIA-N2 non-small cell lung cancer: focusing on an effect of the number of mediastinal lymph node stations involved.

• DOI: 10.1510/icvts.2007.174342
• 发表时间: 2008-08-01
• 期刊: Interactive cardiovascular and thoracic surgery
• 作者: Matsuguma, Haruhisa;Nakahara, Rie;Yokoi, Kohei
• 通讯作者: Yokoi, Kohei

Preoperative evaluation of the depth of chest wall invasion and the extent of combined resections in lung cancer patients

• DOI: 10.1016/j.lungcan.2008.07.006
• 发表时间: 2009-04-01
• 期刊: LUNG CANCER
• 影响因子: 5.3
• 作者: Kawaguchi, Koji;Mori, Shoichi;Yokoi, Kohei
• 通讯作者: Yokoi, Kohei