Therapeutic benefits of intravenous administration of genetically-engineering mesenchymal stem cells on spinal cord injury

基因工程间充质干细胞静脉注射对脊髓损伤的治疗作用

• 批准号: 20591717
• 负责人: NONAKA Tadashi
• 金额: $ 3万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591717/
• 关键词: 移植・再生医療; 再生医学; 神経科学; 脳・神経; 発生・分化

Local injection of mesenchymal stem cells (MSCs) derived from bone marrow has been shown to improve functional outcome in spinal cord injury (SCI). Systemic delivery of MSCs results in therapeutic benefits in a number of central nervous system disorders. In the present study we intravenously administered MSCs derived from bone marrow to study their potential therapeutic effect in a contusive SCI model in the rat. Light microscopic examination revealed that cavitation in the contused spinal cords was less in the MSC-treated rats. Functional outcome was greater in the MSC-treated groups than in sham controls with greatest improvement in the earlier post-contusion infusion times. BDNF-hypersecreting MSC enhanced the therapeutic benefits of MSC on SCI. The availability of autologous MSCs in large number and the potential for systemically delivering cells to target lesion areas without neurosurgical intervention suggests the potential utility of intravenous cell delivery as a prospective therapeutic approach in SCI.

局部注射骨髓间充质干细胞（MSC）已被证明可以改善脊髓损伤（SCI）的功能结果。MSC的全身递送在许多中枢神经系统病症中产生治疗益处。在本研究中，我们静脉注射骨髓来源的间充质干细胞，以研究其在大鼠挫伤性脊髓损伤模型中的潜在治疗作用。光学显微镜检查显示，在挫伤脊髓中的空泡在MSC治疗的大鼠较少。MSC治疗组的功能结局比假手术对照组更好，在挫伤后早期输注时间改善最大。高分泌BDNF的MSC增强了MSC对SCI的治疗效果。大量自体骨髓间充质干细胞的可用性和在没有神经外科干预的情况下将细胞全身递送到靶病变区域的潜力表明静脉内细胞递送作为SCI的前瞻性治疗方法的潜在效用。

项目成果

脊髄損傷に対する培養骨髄幹細胞の静脈内投与 社団法人 日本脳神経外科学会

静脉注射培养的骨髓干细胞治疗脊髓损伤 日本神经外科学会

• 发表时间: 2010
• 作者: Sasaki;et al.;本望修
• 通讯作者: 本望修

Intravenous administration of mesenchymal stem cells derived from bone marrow after contusive spinal cord injury improves functional outcome

• DOI: 10.1016/j.brainres.2010.05.011
• 发表时间: 2010-07-09
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Osaka, Misuzu;Honmou, Osamu;Kocsis, Jeffery D.
• 通讯作者: Kocsis, Jeffery D.

脊髄損傷に対する培養骨髄幹細胞の静脈内投与

静脉注射培养的骨髓干细胞治疗脊髓损伤

• 发表时间: 2010
• 作者: 山中龍也;他;Ogawa T;本望修
• 通讯作者: 本望修

BDNF-hypersecreting human mesenchymal stem cells promote functional recovery, axonal sprouting, and protection of corticospinal neurons after spinal cord injury.

• DOI: 10.1523/jneurosci.2769-09.2009
• 发表时间: 2009-11-25
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Sasaki M;Radtke C;Tan AM;Zhao P;Hamada H;Houkin K;Honmou O;Kocsis JD
• 通讯作者: Kocsis JD

脊髄損傷における骨髄幹細胞の移植による機能回復

骨髓干细胞移植治疗脊髓损伤的功能恢复

• 发表时间: 2009
• 作者: 大阪美鈴;本望修;村上友宏;宝金清博
• 通讯作者: 宝金清博