利用微流控/纳米技术构建血管/神经网络用于再生医学

本课题拟采利用微流控、静电纺丝、细胞图案化、三维血管构建、纳米金颗粒抗菌等技术，研究心肌细胞、血管细胞及神经细胞的生长控制及其相互作用。首先单独构建心肌/神经细胞补片及包含外周神经的三维血管，进而构建具有血管及神经网络的心肌补片，并最终用于诱导实现心肌的再生。神经与血管广泛存在于人体的各个部位，两者相辅相成、互为依托。因此，由于血管与神经网络的广泛存在性，对其相互作用展开深入研究，积累大量相关技术经验，不仅对心脑血管组织再生领域有着重大影响，而且对所有组织和器官的再生都将有重大的意义。

This proposal aims at myocardium regeneration, which combines microfluidics, electrospinning, cell patterning, three dimensional vascular construction, and gold nanoparticle-based antibacterial technology. We focus on the growth control of cardiomyocytes, neurons, vascular endothelial cells, smooth muscle cells and fibroblasts, and interactions between them. We intend to realize myocardium regeneration by fabricating myocardial patch. We plan to first construct the cadiomyocytes/neurons patch and three dimensional blood vessel surrounded by neurons, secondly integrate these two parts to form a patch with vascular and neural network. Neurons and blood vessels wildly exist in various parts of human body, and are always inseparably interconnected and supplementary to each other. These novel approaches will not only have significant impacts on the cardiovascular medicine, but also on the regeneration of most tissues and organs.