Homing of circulating osteogenic progenitors to sites of fracture repair under the low-intensity pulsed ultrasound treatment

低强度脉冲超声治疗下循环成骨祖细胞归巢至骨折修复部位

• 批准号: 20591763
• 负责人: SAITO Tomoyuki
• 金额: $ 3.24万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591763/
• 关键词: 骨折治癒; 低出力超音波; 末梢血由来細胞; ホーミング; 骨形成促進効果; 骨形成促進

A parabiotic model was created by surgically conjoining one transgenic GFP mouse and one wild-type partner. After shared circulation was established, a transverse fracture with periosteal stripping was created in the contralateral diaphysial femur of the wild-type partner. The fracture site was treated with daily exposure of low-intensity pulsed ultrasound (LIPUS). GFP+ cells that gained access to the osteotomy site via the systemic circulation were identified in the bone-forming site. The findings suggested that LIPUS induced homing of circulating osteogenic progenitors to the osteotomy site for possible contribution to new bone formation.

通过手术将一只转基因GFP小鼠和一只野生型伴侣连接起来，建立了一种共生模型。在建立共享循环后，在野生型伴侣的对侧股骨骨干上造成横形骨折并剥离骨膜。骨折部位采用低强度脉冲超声(LIPUS)每日照射治疗。在骨形成部位发现了通过体循环进入截骨部位的GFP+细胞。研究结果表明，LIPUS诱导循环中的成骨祖细胞归巢到截骨部位，可能对新骨形成有贡献。

项目成果

Clinical results and radiographical evaluation of opening wedge high tibial osteotomy for spontaneous osteonecrosis of the knee

• DOI: 10.1007/s00167-008-0698-4
• 发表时间: 2009-04-01
• 期刊: KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
• 影响因子: 3.8
• 作者: Takeuchi, Ryohei;Aratake, Masato;Saito, Tomoyuki
• 通讯作者: Saito, Tomoyuki

Relationship Between Low Bone Mineral Density and Varus Deformity in Postmenopausal Women with Knee Osteoarthritis

• DOI: 10.3899/jrheum.080699
• 发表时间: 2009-03-01
• 期刊: JOURNAL OF RHEUMATOLOGY
• 影响因子: 3.9
• 作者: Akamatsu, Yasushi;Mitsugi, Naoto;Saito, Tomoyuki
• 通讯作者: Saito, Tomoyuki

Effects of Low-Intensity Pulsed Ultrasound on Intramedullary Bone Formation and Homing of Circulating Connective Tissue Progenitors in Fracture with Periosteal Stripping.

低强度脉冲超声对骨膜剥离骨折中髓内骨形成和循环结缔组织祖细胞归巢的影响。

• 发表时间: 2009
• 作者: Kumagai K;Takeuchi R;Ishikawa H;Saito I;Yamaguchi Y;Chiba N;Takagawa S;Saito T.
• 通讯作者: Saito T.

Low-Intensity Pulsed Ultrasound Enhances Fracture Healing and Recruitment of Circulating Osteogenic Progenitors.

低强度脉冲超声增强骨折愈合和循环成骨祖细胞的募集。

• 发表时间: 2010
• 作者: Kumagai K;Takeuchi R;Ishikawa H;Saito I;Yamaguchi Y;Chiba N;Takagawa S;Saito T;熊谷研
• 通讯作者: 熊谷研

Clinical results of a valgus high tibial osteotomy for the treatment of osteoarthritis of the knee and the ipsilateral ankle

• DOI: 10.1016/j.knee.2008.02.002
• 发表时间: 2008-06-01
• 期刊: KNEE
• 影响因子: 1.9
• 作者: Takeuchi, Ryohei;Saito, Tomoyuki;Koshino, Tomihisa
• 通讯作者: Koshino, Tomihisa