Homing of circulating osteogenic progenitors to sites of fracture repair under the low-intensity pulsed ultrasound treatment

低强度脉冲超声治疗下循环成骨祖细胞归巢至骨折修复部位

• 批准号: 20591763
• 负责人: SAITO Tomoyuki
• 金额: $ 3.24万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591763/
• 关键词: 骨折治癒; 低出力超音波; 末梢血由来細胞; ホーミング; 骨形成促進効果; 骨形成促進

A parabiotic model was created by surgically conjoining one transgenic GFP mouse and one wild-type partner. After shared circulation was established, a transverse fracture with periosteal stripping was created in the contralateral diaphysial femur of the wild-type partner. The fracture site was treated with daily exposure of low-intensity pulsed ultrasound (LIPUS). GFP+ cells that gained access to the osteotomy site via the systemic circulation were identified in the bone-forming site. The findings suggested that LIPUS induced homing of circulating osteogenic progenitors to the osteotomy site for possible contribution to new bone formation.

通过手术结合了一只转基因GFP小鼠和一只野生型伴侣来创建一个抛物性模型。建立共同的循环后，在野生型伴侣的对侧dia肌股骨中产生了带有骨膜剥离的横向骨折。每天暴露于低强度脉冲超声（Lipus），对断裂部位进行处理。在骨形成部位发现了通过全身循环获得截骨部位的GFP+细胞。研究结果表明，Lipus诱导将循环成骨祖细胞归为截骨部位，以便对新骨形成产生贡献。

项目成果

A mid term comparison of open wedge high tibial osteotomy vs unicompartmental knee arthroplasty for medial compartment osteoarthritis of the knee.

• DOI: 10.1186/1749-799x-5-65
• 发表时间: 2010-08-30
• 期刊: Journal of orthopaedic surgery and research
• 影响因子: 2.6
• 作者: Takeuchi R;Umemoto Y;Aratake M;Bito H;Saito I;Kumagai K;Sasaki Y;Akamatsu Y;Ishikawa H;Koshino T;Saito T
• 通讯作者: Saito T

Low-Intensity Pulsed Ultrasound Stimulates Intramedullary Bone Formation and Homing of Circulating Connective Tissue Progenitors at Fracture Site with Periosteal Stripping in Mice.

低强度脉冲超声通过小鼠骨膜剥离刺激骨折部位的髓内骨形成和循环结缔组织祖细胞的归巢。

• 发表时间: 2009
• 作者: 熊谷研;竹内良平;荒武正人;斎藤泉;尾藤晴彦;佐々木洋平;齋藤知行;吉田宗人;熊谷研;吉田宗人;熊谷 研
• 通讯作者: 熊谷 研

Effects of Low-Intensity Pulsed Ultrasound on Intramedullary Bone Formation and Homing of Circulating Connective Tissue Progenitors in Fracture with Periosteal Stripping.

低强度脉冲超声对骨膜剥离骨折中髓内骨形成和循环结缔组织祖细胞归巢的影响。

• 发表时间: 2009
• 作者: Kumagai K;Takeuchi R;Ishikawa H;Saito I;Yamaguchi Y;Chiba N;Takagawa S;Saito T.
• 通讯作者: Saito T.

Low-Intensity Pulsed Ultrasound Enhances Fracture Healing and Recruitment of Circulating Osteogenic Progenitors.

低强度脉冲超声增强骨折愈合和循环成骨祖细胞的募集。

• 发表时间: 2010
• 作者: Kumagai K;Takeuchi R;Ishikawa H;Saito I;Yamaguchi Y;Chiba N;Takagawa S;Saito T;熊谷研
• 通讯作者: 熊谷研

Bone formation and homing of circulating connective tissue progenitors at fracture site were enhanced by low-intensity pulsed ultrasound.

低强度脉冲超声增强了骨折部位的骨形成和循环结缔组织祖细胞的归巢。

• 发表时间: 2010
• 作者: Kumagai K;Takeuchi R;Ishikawa H;Yamaguchi Y;Fujisawa T;Kuniya T;Takagawa S;Saito T.
• 通讯作者: Saito T.