Neuroprotective effect of fructose 1,6-diphosphate : a 31P-NMR study

果糖1,6-二磷酸酯是糖酵解的中间底物。它是一种已知的神经保护剂，作为能量代谢物和自由基清除剂。采用31p -核磁共振波谱（^<31>P-NMR）研究FDP的神经保护能力。用31P-NMR测定缺血再灌注损伤时的能量代谢状态。脑切片经5 mM FDP处理后，磷酸肌酸（活细胞中ATP的能量缓冲液）的回收率显著提高。我们的一系列实验，包括31P-NMR和电子自旋共振谱，表明FDP不仅作为糖酵解的能量代谢物，而且作为自由基清除剂，具有神经保护剂的作用。

Fructose 1,6-diphophate is an intermediate substrate of glycolysis. It is a known neuroprotectant and acts as energy metabolite and a radical scavenger. The neuroprotective capacity of FDP was studied using 31P-nuclear magnetic resonance spectroscopy (^<31>P-NMR). The state of energy metabolism during ischemia-reperfusion insult was evaluated by 31P-NMR. The recovery of phosphocreatine, energetic buffer of ATP in living cells, was significantly higher when brain slices were superfused with 5 mM FDP. The series of our experiments, including 31P-NMR and electron spin resonance spectroscopy, indicated that FDP functions as a neuroprotectant not only as an energy metabolite of glycolysis but also as a radical scavenger.