Study on the expression of ion channels in inflammation-induced uterine smooth muscle elucidating the mechanisms of preterm delivery.

炎症诱导的子宫平滑肌离子通道表达的研究阐明早产机制。

• 批准号: 20591917
• 负责人: YAMAOKA Kaoru
• 金额: $ 3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591917/
• 关键词: ATP受容体; P2X7チャンネル; 子宮平滑筋; 早産; 周産期医学; パッチクランプ

In pregnant rat myometrium, P2X4 and P2X7 receptors, one of ATP-sensitive non-selective cation channels (NSCC), were suggested to be highly expressed by the quantitative evaluation of mRNA s and proteins. NSCC currents observed in freshly isolated cells from pregnant rat myometrium resembles to those from COS7 cells where P2X7 receptors were transfected, indicating P2X7 receptor in rat myometrium play a major role in conducting NSCC currents. It may be suggested that C^<2+> influx through P2X7 may trigger labor contraction. In uterus of inflammatory model rats induced by application of LPS (lipopolysaccharide), P2X7 receptors were highly expressed. Thus, these results indicate that stimulation of P2X7 receptor expression in rat myometrium induced by inflammation may lead to preterm delivery. Further study on this subject should contribute to the elucidation of precise mechanisms of preterm delivery as well as development of new therapies and new technologies of prevention against preterm delivery.

通过mRNA和蛋白质的定量测定，提示妊娠大鼠子宫肌层中存在ATP敏感的非选择性阳离子通道（NSCC）之一的P2X4和P2X7受体的高表达。在从妊娠大鼠子宫肌层新鲜分离的细胞中观察到的NSCC电流类似于来自转染P2X7受体的COS7细胞的电流，表明大鼠子宫肌层中的P2X7受体在传导NSCC电流中起主要作用。提示C^<2+>通过P2X7内流可引起产程收缩。在LPS（脂多糖）诱导的炎症模型大鼠子宫中，P2X7受体高表达。因此，这些结果表明，炎症诱导的大鼠子宫肌层中P2X7受体表达的刺激可能导致早产。对这一问题的进一步研究将有助于阐明早产的确切机制，以及开发预防早产的新疗法和新技术。

项目成果

妊娠ラット子宮平滑筋細胞において主として機能しているATP受容体はP2X7チャンネルである

妊娠大鼠子宫平滑肌细胞中起作用的主要 ATP 受体是 P2X7 通道。

• 发表时间: 2009
• 作者: Urabe S;Miyoshi H;el al.;三好博史,占部智,谷川美穂,島筒里香子,藤原久也,工藤美樹
• 通讯作者: 三好博史,占部智,谷川美穂,島筒里香子,藤原久也,工藤美樹

妊娠ラット炎症モデルにおける子宮平滑筋非選択性陽イオンチャンネルの発現と早産との関連についての検討

孕鼠炎症模型子宫平滑肌非选择性阳离子通道表达与早产关系的探讨

• 发表时间: 2008
• 作者: 占部智;三好博史;島筒里香子;藤原久也;工藤美樹
• 通讯作者: 工藤美樹

硫酸マグネシウムの薬理作用

硫酸镁的药理作用

• 发表时间: 2008
• 作者: 占部 智;三好博史;島筒里香子;藤原久也;工藤美樹;三好博史
• 通讯作者: 三好博史

Functional expression of purinergic P2X7 receptors in pregnant rat myometrium

• DOI: 10.1152/ajpregu.00507.2009
• 发表时间: 2010-04-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
• 影响因子: 2.8
• 作者: Miyoshi, Hiroshi;Yamaoka, Kaoru;Kudo, Yoshiki
• 通讯作者: Kudo, Yoshiki

Enhanced expression of P2X4 and P2X7 purinergic receptors in the myometrium of pregnant rats in preterm delivery models..

早产模型妊娠大鼠子宫肌层 P2X4 和 P2X7 嘌呤能受体表达增强。

• 发表时间: 2009
• 期刊: Reproductive Sciences 16
• 作者: Urabe S;Miyoshi H;Yamaoka;K.;Kudo Y.
• 通讯作者: Kudo Y.