Development of anti-tumor gene therapy for retinoblastoma using Sendai virus vector and IFNB gene.

目的：探讨携带干扰素-β基因的M基因缺陷仙台病毒载体(SEV/Δ、M-干扰素-β)抗肿瘤基因治疗视网膜母细胞瘤的可能性。SeV/ΔM载体在视网膜母细胞瘤细胞系中的基因转导效率较低，但在视网膜色素上皮来源的细胞系中可介导高效的基因转移。SEV/ΔM载体介导的干扰素-β在视网膜母细胞瘤中的过表达抑制了视网膜母细胞瘤细胞的生长，提示应用SEV/βM载体眼内转移干扰素-Δ基因可能是预防视网膜母细胞瘤患者肿瘤生长的一种有用的策略。

We investigated weather anti-tumor gene therapy using M gene deficient Sendai virus vector encoding IFN-β gene (SeV/ΔM-IFN-β) has therapeutic potentials for the treatment of retinoblastoma. Whereas the gene transduction efficiency of SeV/ΔM vector is low in retinoblastoma cell lines, it mediates efficient gene transfer in a retinal pigment epithelium (RPE)-derived cell line. SeV/ΔM-mediated overexpression of IFN-β in RPE suppressed the growth of retinoblastoma cells, suggesting that intraocular IFN-β gene transfer using SeV/ΔM vector may be a useful strategy for preventing tumor growth in retinoblastoma patients.